Department of Hepatobiliary Surgery, Peking University People's Hospital, Beijing 100044, China.
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing Mass Spectrum Center, Beijing 100190, China.
Int J Mol Sci. 2017 Nov 28;18(12):2550. doi: 10.3390/ijms18122550.
The aim of this study was to characterize the disorder of lipid metabolism in hepatocellular carcinoma (HCC). HCC is a worldwide disease. The research into the disorder of lipid metabolism in HCC is very limited. Study of lipid metabolism in liver cancer tissue may have the potential to provide new insight into HCC mechanisms.
A lipidomics study of HCC based on Ultra high performance liquid chromatography-electronic spray ionization-QTOF mass spectrometer (UPLC-ESI-QTOF MS) and Matrix assisted laser desorption ionization-fourier transform ion cyclotron resonance mass spectrometer (MALDI-FTICR MS) was performed.
Triacylglycerols (TAGs) with the number of double bond (DB) > 2 (except 56:5 and 56:4 TAG) were significantly down-regulated; conversely, others (except 52:2 TAG) were greatly up-regulated in HCC tissues. Moreover, the more serious the disease was, the higher the saturated TAG concentration and the lower the polyunsaturated TAG concentration were in HCC tissues. The phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI) were altered in a certain way. Sphingomyelin (SM) was up-regulated and ceramide (Cer) were down-regulated in HCC tissues.
To our knowledge, this is the first such report showing a unique trend of TAG, PC, PE and PI. The use of polyunsaturated fatty acids, like eicosapentanoic and docosahexanoic acid, as supplementation, proposed for the treatment of Non-alcoholic steatohepatitis (NASH), may also be effective for the treatment of HCC.
本研究旨在描述肝细胞癌（HCC）中的脂质代谢紊乱。HCC 是一种全球性疾病，对 HCC 中脂质代谢紊乱的研究非常有限。对肝癌组织中脂质代谢的研究可能有潜力为 HCC 机制提供新的见解。
采用超高效液相色谱-电喷雾电离-四极杆飞行时间质谱（UPLC-ESI-QTOF MS）和基质辅助激光解吸电离-傅里叶变换离子回旋共振质谱（MALDI-FTICR MS）对 HCC 进行脂质组学研究。
具有两个以上双键（DB）的TAG（56:5 和 56:4 TAG 除外）明显下调；相反，其他（52:2 TAG 除外）在 HCC 组织中显著上调。此外，疾病越严重，HCC 组织中饱和 TAG 浓度越高，多不饱和 TAG 浓度越低。PC、PE 和 PI 以某种方式发生改变。SM 在 HCC 组织中上调，Cer 下调。
据我们所知，这是第一个显示 TAG、PC、PE 和 PI 独特趋势的此类报告。多不饱和脂肪酸，如二十碳五烯酸和二十二碳六烯酸，作为补充剂，用于治疗非酒精性脂肪性肝炎（NASH），也可能对 HCC 的治疗有效。
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