School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR China
Int J Mol Sci. 2017 Nov 24;18(12):2516. doi: 10.3390/ijms18122516.
Aptamers are short nucleic acid sequences capable of specific, high-affinity molecular binding. They are isolated via SELEX (Systematic Evolution of Ligands by Exponential Enrichment), an evolutionary process that involves iterative rounds of selection and amplification before sequencing and aptamer characterization. As aptamers are genetic in nature, bioinformatic approaches have been used to improve both aptamers and their selection. This review will discuss the advancements made in several enclaves of aptamer bioinformatics, including simulation of aptamer selection, fragment-based aptamer design, patterning of libraries, identification of lead aptamers from high-throughput sequencing (HTS) data and in silico aptamer optimization.
适体是能够进行特异性、高亲和力分子结合的短核酸序列。它们通过 SELEX（指数富集的配体系统进化）分离，这是一个涉及反复选择和扩增的进化过程，然后进行测序和适体表征。由于适体本质上是遗传的，因此已经使用生物信息学方法来改进适体及其选择。本综述将讨论适体生物信息学的几个领域的进展，包括适体选择的模拟、基于片段的适体设计、文库的模式化、从高通量测序 (HTS) 数据中鉴定先导适体以及适体的计算机优化。
