Bacher J, Kassianides C, Moskal T J, Mathews D M, Hoofnagle J H
Division of Research Services, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD.
Lab Anim Sci. 1989 Jan;39(1):67-8.
The duck hepatitis B virus (DHBV), a member of the hepadna-virus group, has become a useful animal model for exploring important aspects in this family of viruses such as viral replication, course of infection, and the response to antiviral therapy. In chronically DHBV infected ducks, repeated analyses of liver tissue are important in defining the degree of viral replication and liver injury. We describe a technique for repeated liver biopsy using a Keyes skin punch biopsy. This technique provided sufficient quantities of liver tissue for serial analyses with minimal hemorrhage in 18 Pekin ducks. This procedure offers a safe and reliable method of obtaining serial liver biopsies.
鸭乙型肝炎病毒（DHBV）是嗜肝DNA病毒科的一员，已成为探索该病毒家族重要方面（如病毒复制、感染过程和对抗病毒治疗的反应）的有用动物模型。在慢性感染DHBV的鸭中，对肝组织进行反复分析对于确定病毒复制程度和肝损伤程度很重要。我们描述了一种使用凯斯皮肤打孔活检进行反复肝活检的技术。该技术为18只北京鸭的系列分析提供了足够数量的肝组织，且出血极少。该程序提供了一种安全可靠的获取系列肝活检的方法。
