通过恒流灌注评估啮齿动物的房水动力学

Assessment of Aqueous Humor Dynamics in the Rodent by Constant Flow Infusion.

作者信息

Millar J Cameron, Phan Tien N, Pang Iok-Hou

机构信息

North Texas Eye Research Institute, University of North Texas Health Science Center, 3500 Camp Bowie Boulevard, Fort Worth, TX, 76107, USA.

Department of Pharmaceutical Sciences, College of Pharmacy, University of North Texas Health Science Center, 3500 Camp Bowie Boulevard, Fort Worth, TX, 76107, USA.

出版信息

Methods Mol Biol. 2018;1695:109-120. doi: 10.1007/978-1-4939-7407-8_11.

DOI:10.1007/978-1-4939-7407-8_11

PMID:29190023

Abstract

Mice and rats are being increasingly used in glaucoma research and much useful data have been generated from them. One aspect of using these animals for this purpose involves assessment of aqueous humor dynamics. Several techniques have been described in the literature for the determination of one or more of these parameters in rodents, in both living animals and eyes perfused ex vivo. Here, we describe the practical details for a technique for the determination of all principal parameters of aqueous humor dynamics (intraocular pressure (IOP), aqueous humor formation rate (Fin), uveoscleral outflow rate (Fu), aqueous outflow facility (C), and episcleral venous pressure (Pe)) in the living rat and mouse eye, in a single experimental session.

摘要

小鼠和大鼠在青光眼研究中的应用越来越广泛，并由此产生了许多有用的数据。将这些动物用于此目的的一个方面涉及房水动力学评估。文献中已经描述了几种用于测定啮齿动物体内这些参数中的一个或多个的技术，包括活体动物和离体灌注眼。在此，我们描述了一种在活体大鼠和小鼠眼中单次实验测定房水动力学所有主要参数（眼压（IOP）、房水生成率（Fin）、葡萄膜巩膜流出率（Fu）、房水流出易度（C）和巩膜静脉压（Pe））的技术的实际细节。