应变和年龄对小鼠房水动力学的影响。

Strain and Age Effects on Aqueous Humor Dynamics in the Mouse.

作者信息

Millar J Cameron, Phan Tien N, Pang Iok-Hou, Clark Abbot F

机构信息

North Texas Eye Research Institute (NTERI), University of North Texas Health Science Center, Fort Worth, Texas, United States 2Department of Cell Biology & Immunology, University of North Texas Health Science Center, Fort Worth, Texas, United States.

出版信息

Invest Ophthalmol Vis Sci. 2015 Sep;56(10):5764-76. doi: 10.1167/iovs.15-16720.

DOI:10.1167/iovs.15-16720

PMID:26325415

Abstract

PURPOSE

We evaluated differences in aqueous humor dynamics (AHD) among several mouse strains within younger and older age groups.

METHODS

Albino (A/J, BALB/cJ) and pigmented (C3H/HeJ, C57-BL/6J) mice (young [2½-4½ months] and aged [10-12 months]) were studied. Intraocular pressure (IOP) was measured. In cannulated eyes, episcleral venous pressure (Pe) was assessed (blood reflux). Other AHD parameters (outflow facility [C], aqueous humor formation rate [Fin]) were assessed (constant flow infusion). Uveoscleral outflow rate (Fu) was obtained by calculation (Fu(calc)) using the modified Goldmann equation, and in additional eyes (for comparison), by FITC-dextran perfusion (Fu(FITC-dex)).

RESULTS

Intraocular pressure was higher in pigmented strains, but did not exhibit age-dependence, except in the C57-BL/6J strain. Fu(calc) decreased with age in BALB/cJ (↓83.3%), C3H/HeJ (↓78.0%), and C57-BL/6J (↓85.0%) strains. In the A/J strain, Fu(calc) decreased with age (↓70.0%), but not significantly. Fin decreased with age in the C3H/HeJ (↓53.6%) strain. In C57-BL/6J and A/J strains, Fin decreased with age, but not significantly. C in the BALB/cJ strain increased with age (↑62.5%). In C3H/HeJ and C57-BL/6J strains, C increased with age, but not significantly. Episcleral venous pressure ranged from 6.0 to 6.6 mm Hg (albino strains) to 8.5 to 8.9 mm Hg (pigmented strains). Pe was not age dependent, but was higher in pigmented animals.

CONCLUSIONS

In mouse, Fu and Fin diminish with age. C tends to increase as animals progress to middle life. There are strain differences in Fu, IOP, C, Fin, and Pe. The current findings provide an important foundation for comparisons among different strains in different study reports.

摘要

目的

我们评估了年轻和老年年龄组中几种小鼠品系之间房水动力学（AHD）的差异。

方法

研究了白化小鼠（A/J、BALB/cJ）和有色小鼠（C3H/HeJ、C57-BL/6J）（年轻[2.5 - 4.5个月]和老年[10 - 12个月]）。测量眼压（IOP）。在插管眼中，评估巩膜静脉压（Pe）（血液反流）。评估其他AHD参数（流出易度[C]、房水生成率[Fin]）（恒流灌注）。使用改良的戈德曼方程通过计算获得葡萄膜巩膜流出率（Fu）（Fu(calc)），并在另外的眼睛中（用于比较）通过异硫氰酸荧光素 - 葡聚糖灌注获得（Fu(FITC-dex)）。

结果

有色品系的眼压较高，但除C57-BL/6J品系外不表现出年龄依赖性。在BALB/cJ（下降83.3%）、C3H/HeJ（下降78.0%）和C57-BL/6J（下降85.0%）品系中，Fu(calc)随年龄下降。在A/J品系中，Fu(calc)随年龄下降（下降70.0%），但不显著。在C3H/HeJ（下降53.6%）品系中，Fin随年龄下降。在C57-BL/6J和A/J品系中，Fin随年龄下降，但不显著。BALB/cJ品系中的C随年龄增加（增加62.5%）。在C3H/HeJ和C57-BL/6J品系中，C随年龄增加，但不显著。巩膜静脉压范围为6.0至6.6 mmHg（白化品系）至8.5至8.9 mmHg（有色品系）。Pe不依赖于年龄，但在有色动物中较高。