Sanches Marcella, Assis Lívia, Criniti Cyntia, Fernandes Danilo, Tim Carla, Renno Ana Claudia Muniz
Department of Bioscience, Federal University of São Paulo, R. Silva Jardim, 136, Vila Mathias, Santos, SP, 11015-020, Brazil.
Department of Biomedical Engineering, Brazil University, São Paulo, Brazil.
Lasers Med Sci. 2018 Apr;33(3):549-557. doi: 10.1007/s10103-017-2401-9. Epub 2017 Dec 1.
The aim of this study was to compare the effects of combined treatment with chondroitin sulfate and glucosamine sulfate (CS/Gl) and photobiomodulation (PBM) on the degenerative process related to osteoarthritis (OA) in the articular cartilage in rats. Forty male Wistar rats were randomly divided into four groups: OA control group (CG); OA animals submitted to PBM treatment (PBM); OA animals submitted to CS/Gl treatment (CS/Gl); OA submitted to CS/GS associated with PBM treatments (GS/Gl + PBM). The CS/Gl started 48 h after the surgery, and they were performed for 29 consecutive days. Moreover, PBM was performed after the CS/Gl administration on the left joint. Morphological characteristics and immunoexpression of interleukin 10 (IL-10) and 1 beta (IL-1β) and collagen type II (Col II) of the articular cartilage were evaluated. The results showed that all treated groups (CS/Gl and PBM) presented attenuation signs of degenerative process (measured by histopathological analysis) and lower density chondrocytes [PBM (p = 0.0017); CS/Gl (p = 0.0153) and CS/Gl + PBM (p = 0.002)]. Additionally, CS/Gl [associated (p = 0.0089) or not with PBM (p = 0.0059)] showed significative lower values for OARSI grade evaluation. Furthermore, CS/GS + PBM decreased IL-1β protein expression (p = 0.0359) and increased IL-10 (p = 0.028) and Col II imunoexpression (p = 0.0204) compared to CG. This study showed that CS/Gl associated with PBM was effective in modulating inflammatory process and preventing the articular tissue degradation in the knees OA rats.
本研究旨在比较硫酸软骨素和硫酸氨基葡萄糖联合治疗（CS/Gl）与光生物调节（PBM）对大鼠关节软骨中与骨关节炎（OA）相关的退变过程的影响。40只雄性Wistar大鼠被随机分为四组：OA对照组（CG）；接受PBM治疗的OA动物（PBM）；接受CS/Gl治疗的OA动物（CS/Gl）；接受CS/Gl联合PBM治疗的OA动物（GS/Gl + PBM）。CS/Gl在手术后48小时开始，连续进行29天。此外，在CS/Gl给药后对左侧关节进行PBM治疗。评估关节软骨的形态学特征、白细胞介素10（IL-10）、白细胞介素1β（IL-1β）和II型胶原（Col II）的免疫表达。结果表明，所有治疗组（CS/Gl和PBM）均呈现退变过程的减轻迹象（通过组织病理学分析测量）以及较低密度的软骨细胞[PBM组（p = 0.0017）；CS/Gl组（p = 0.0153）和CS/Gl + PBM组（p = 0.002）]。此外，CS/Gl组[联合PBM组（p = 0.0089）或未联合PBM组（p = 0.0059）]在骨关节炎研究学会国际分会（OARSI）分级评估中显示出显著更低的值。此外，与CG组相比，CS/GS + PBM组降低了IL-1β蛋白表达（p = 0.0359），增加了IL-10（p = 0.028）和Col II免疫表达（p = 0.0204）。本研究表明，CS/Gl联合PBM可有效调节炎症过程并预防膝骨关节炎大鼠关节组织的降解。
