Shababdoust A, Ehsani M, Shokrollahi P, Zandi M
Plastics Department, Iran Polymer and Petrochemical Institute, Tehran, P.O. Box 14965/115, Iran.
Biomaterials Department, Iran Polymer and Petrochemical Institute, Tehran, P.O. Box 14965/115, Iran.
Prog Biomater. 2018 Mar;7(1):23-33. doi: 10.1007/s40204-017-0079-5. Epub 2017 Dec 1.
Two series of polyurethane (PU), based on polycaprolactone (PCL) as soft segments with two different molecular weights (2000 and 530 Da), and hexamethylene diisocyanate (HDI) and 1,4-butandiol (BDO) as hard segments were synthesized to fabricate curcumin-loaded electrospun nanofibrous PCL-based PU substrate. Chemical structures of the synthesized PUs were characterized by FTIR and NMR spectroscopy techniques. The thermal properties were analyzed by differential scanning calorimetry (DSC) and surface hydrophilicity was studied by static contact angle and bulk hydrophilicity was evaluated by water uptake test. Thereafter, bead-free PU nanofiberous substrate containing curcumin was fabricated by electrospinning and morphology of the mats was observed by scanning electron microscopy (SEM). Mechanical properties of the electrospun mats in comparison with polymeric films were assessed by a universal test machine. The in vitro release of curcumin was studied by UV-Vis spectroscopy. The optical density of the bacterial solutions was used to evaluate the antibacterial activity of the curcumin-loaded nanofibrous mats against Escherichia coli (E-coli ATCC: 25922). The results showed that curcumin-loaded PU synthesized by PCL with molecular weight of 2000 Da displayed better mechanical properties as well as better antibacterial properties in wound dressing application.
合成了两个系列的聚氨酯（PU），其以聚己内酯（PCL）作为软段，具有两种不同分子量（2000和530 Da），并以六亚甲基二异氰酸酯（HDI）和1,4 - 丁二醇（BDO）作为硬段，用于制备负载姜黄素的基于聚己内酯的电纺纳米纤维PU基质。通过傅里叶变换红外光谱（FTIR）和核磁共振光谱（NMR）技术对合成的聚氨酯的化学结构进行了表征。通过差示扫描量热法（DSC）分析热性能，并通过静态接触角研究表面亲水性，通过吸水率测试评估整体亲水性。此后，通过静电纺丝制备了含姜黄素的无珠PU纳米纤维基质，并通过扫描电子显微镜（SEM）观察了毡垫的形态。通过万能试验机评估了与聚合物膜相比电纺毡垫的机械性能。通过紫外 - 可见光谱研究了姜黄素的体外释放。细菌溶液的光密度用于评估负载姜黄素的纳米纤维毡垫对大肠杆菌（E - coli ATCC：25922）的抗菌活性。结果表明，由分子量为2000 Da的PCL合成的负载姜黄素的PU在伤口敷料应用中表现出更好的机械性能以及更好的抗菌性能。
