Rezaei Tavirani Mostafa, OkHOVATIAN Farshad, Zamanian Azodi Mona, Rezaei Tavirani Majid
Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Physiotherapy Research Centre, School of Rehabilitation, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iran J Child Neurol. 2017 Fall;11(4):7-14.
Duchenne muscular dystrophy (DMD) is one of the mortal diseases, subjected to study in terms of molecular investigation. In this study, the protein interaction map of this muscle-wasting condition was generated to gain a better knowledge of interactome profile of DMD.
MATERIALS & METHODS: Applying Cytoscape and String Database, the protein-protein interaction network was constructed and the gene ontology of the constructed network was analyzed for biological process, molecular function, and cellular component annotations.
Among 100 proteins related to DMD, dystrophin, utrophin, caveolin 3, and myogenic differentiation 1 play key roles in DMD network. In addition, the gene ontology analysis showed that regulation processes, kinase activity, and sarcoplasmic reticulum were the highlighted biological processes, molecular function, and cell component enrichments respectively for the proteins related to DMD.
The central proteins and the enriched ontologies can be suggested as possible prominent agents in DMD; however, the validation studies may be required.
杜氏肌营养不良症（DMD）是一种致命疾病，需从分子层面进行研究。在本研究中，构建了这种肌肉萎缩病症的蛋白质相互作用图谱，以更好地了解DMD的相互作用组概况。
应用Cytoscape和String数据库构建蛋白质-蛋白质相互作用网络，并对构建网络的基因本体进行生物学过程、分子功能和细胞成分注释分析。
在与DMD相关的100种蛋白质中，抗肌萎缩蛋白、抗肌萎缩蛋白聚糖、小窝蛋白3和生肌分化因子1在DMD网络中起关键作用。此外，基因本体分析表明，调控过程、激酶活性和肌浆网分别是与DMD相关蛋白质突出的生物学过程、分子功能和细胞成分富集项。
核心蛋白和富集的本体可能是DMD中可能的重要因子；然而，可能需要进行验证研究。
