Motayagheni Negar
Wake Forest Institute for Regenerative Medicine, 391 Technology Way, Medical Center Boulevard, Winston-Salem, NC 27157, USA.
MethodsX. 2017 Nov 14;4:508-512. doi: 10.1016/j.mex.2017.11.004. eCollection 2017.
Oscar Langendorff introduced the first method for isolating a heart with contractile activity in 1895. Since then, the Langendorff method has remained a powerful technique in cardiac research and has led to major advances in medicine. The primary goal of the Langendorff method is to provide an isolated heart with oxygen and metabolites via a cannula inserted into the aorta. The Langendorff heart is a complex in vitro technique used primarily in pharmacological and physiological research that allows the evaluation of multiple cardiac hemodynamic parameters including, but not limited to, contractility and heart rate. This article will first provide a brief background of the Langendorff method as well as details regarding organ isolation. Finally, the article will discuss the benefits of a new technique for hanging the isolated heart aorta and the benefits of this technique over traditional methods.
1895年，奥斯卡·兰根多夫（Oscar Langendorff）介绍了第一种分离具有收缩活性心脏的方法。从那时起，兰根多夫方法一直是心脏研究中的一项强大技术，并推动了医学的重大进展。兰根多夫方法的主要目标是通过插入主动脉的插管为离体心脏提供氧气和代谢物。兰根多夫心脏是一种主要用于药理学和生理学研究的复杂体外技术，它允许评估多个心脏血流动力学参数，包括但不限于收缩力和心率。本文将首先简要介绍兰根多夫方法的背景以及器官分离的细节。最后，本文将讨论一种悬挂离体心脏主动脉的新技术的优点以及该技术相对于传统方法的优势。
