暴露于不同氟化物浓度环境下学生的 ENAM 基因变异。
ENAM Gene Variation in Students Exposed to Different Fluoride Concentrations.
机构信息
Dental Sciences, Autonomous Metropolitan University Xochimilco (UAM), Calzada del Hueso 1100, Mexico City 04900, Mexico.
Department of Health Care, Autonomous Metropolitan University Xochimilco (UAM), Calzada del Hueso 1100, Mexico City 04900, Mexico.
出版信息
Int J Environ Res Public Health. 2020 Mar 12;17(6):1832. doi: 10.3390/ijerph17061832.
UNLABELLED
The ENAM gene is important in the formation of tooth enamel; an alteration can affect the lengthening of the crystals, and the thickness in enamel. The objective was to determine the presence of the single nucleotide variant (SNV) rs12640848 of the ENAM gene in students exposed to different concentrations of fluoride.
METHODS
A cross-sectional study was conducted on students exposed to high concentrations of fluoride in the city of Durango which were divided according to the severity of fluorosis and dental caries. Genotype determination was performed by DNA sequencing. The relationship between the severity of dental fluorosis and the allele distribution was determined by the Fisher's exact and Kruskal-Wallis tests.
RESULTS
Seventy-one students were included for the sequencing. In the different allelic variations, for the normal genotype AA/TT, the control group presented 75%, for the AG/TC variation, 70.8% in the TF ≤ 4 group, 65% in TF ≥ 5, and 16.7% in TF = 0; with respect to GG/CC variation, 12.5% in TF ≤ 4, 22% in TF ≥ 5, and 8.3% in TF = 0 group ( = 0.000).
CONCLUSION
The ENAM gene showed an association in the population exposed to different concentrations of fluoride.
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ENAM 基因在牙釉质形成中很重要；其改变会影响晶体的长度和牙釉质的厚度。目的是确定暴露于不同氟浓度的学生的 ENAM 基因单核苷酸变异（SNV）rs12640848 的存在情况。
方法
对暴露于杜兰戈市高氟浓度的学生进行了一项横断面研究，根据氟中毒和龋齿的严重程度进行了分组。通过 DNA 测序进行基因型测定。通过 Fisher 确切检验和 Kruskal-Wallis 检验确定牙釉质氟中毒严重程度与等位基因分布之间的关系。
结果
对 71 名学生进行了测序。在不同的等位基因变异中，对于正常基因型 AA/TT，对照组为 75%，对于 AG/TC 变异，TF≤4 组为 70.8%，TF≥5 组为 65%，TF=0 组为 16.7%；对于 GG/CC 变异，TF≤4 组为 12.5%，TF≥5 组为 22%，TF=0 组为 8.3%（ = 0.000）。
结论
在暴露于不同氟浓度的人群中，ENAM 基因表现出相关性。
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