Metoui Rafika, Bouajila Jalloul, Znati Mansour, Cazaux Sylvie, Neffati Mohamed, Akrout Ahmed
Range Ecology Laboratory, University of Gabès, Institute of Arid Lands, 4119 Medenine, Tunisia.
Laboratory of IMRCP UMR CNRS 5623, Faculty of Pharmacy of Toulouse, University of Toulouse, University of Paul-Sabatier, F-31062 Toulouse, France.
Cell Mol Biol (Noisy-le-grand). 2017 Nov 30;63(11):86-91. doi: 10.14715/cmb/2017.63.11.15.
Four flavones were isolated from dried leaves of Artemisia campestris L. 2',4',5,7-tetrahydroxy-5',6-dimethoxyflavone, eupatilin and dimethoxycentaureidin were reported for the first time in this species whereas cirsiliol was previously identified but it was isolated for the first time. Their structures were elucidated by spectroscopic methods, including 1D and 2D NMR experiments and mass spectrometry analysis. In addition, all isolated flavones were evaluated for their antioxidant, anti-inflammatory, anti-superoxide dismutase, anti-xanthine oxidase and cytotoxic activities. The results showed that all isolated compounds exhibited potent anti-xanthine oxidase activity with IC50 ranging from 3.3 to 6.8 µM, which was higher than that of the control compound allopurinol (8.2 ± 0.6 µM). In addition, cirsiliol was found to be the most cytotoxic against OVCAR-3, IGROV-1and HCT-116 cell lines at 15µM, with inhibition percentage values of 53.7, 48.8 and 40.9%, respectively. All compounds also showed weak to moderate anti-inflammatory and anti-superoxide dismutase activities.
从野艾蒿的干燥叶片中分离出了四种黄酮。2',4',5,7-四羟基-5',6-二甲氧基黄酮、灯盏乙素和二甲氧基矢车菊素在该物种中首次被报道，而cirsiliol之前已被鉴定，但此次是首次分离得到。通过光谱方法，包括一维和二维核磁共振实验以及质谱分析对它们的结构进行了阐明。此外，对所有分离出的黄酮进行了抗氧化、抗炎、抗超氧化物歧化酶、抗黄嘌呤氧化酶和细胞毒性活性评估。结果表明，所有分离出的化合物均表现出较强的抗黄嘌呤氧化酶活性，IC50范围为3.3至6.8μM，高于对照化合物别嘌呤醇（8.2±0.6μM）。此外，发现cirsiliol在15μM时对OVCAR-3、IGROV-1和HCT-116细胞系的细胞毒性最大，抑制率分别为53.7%、48.8%和40.9%。所有化合物还表现出弱至中等程度的抗炎和抗超氧化物歧化酶活性。
