Otterpohl Karla L, Hart Ryan G, Evans Claire, Surendran Kameswaran, Chandrasekar Indra
Enabling Technologies Group - Sanford Research, Sioux Falls, South Dakota, USA.
Molecular Pathology Core, Sanford Research, Sioux Falls, South Dakota, USA.
Physiol Rep. 2017 Dec;5(23). doi: 10.14814/phy2.13513.
The diverse epithelial cell types of the kidneys are segregated into nephron segments and the collecting ducts in order to endow each tubular segment with unique functions. The rich diversity of the epithelial cell types is highlighted by the unique membrane channels and receptors expressed within each nephron segment. Our previous work identified a critical role for Myh9 and Myh10 in mammalian endocytosis. Here, we examined the expression patterns of Nonmuscle myosin 2 (NM2) heavy chains encoded by , , and in mouse kidneys as these genes may confer unique nephron segment-specific membrane transport properties. Interestingly, we found that each segment of the renal tubules predominately expressed only two of the three NM2 isoforms, with isoform-specific subcellular localization, and different levels of expression within a nephron segment. Additionally, we identify Myh14 to be restricted to the intercalated cells and Myh10 to be restricted to the principal cells within the collecting ducts and connecting segments. We speculate that the distinct expression pattern of the NM2 proteins likely reflects the diversity of the intracellular trafficking machinery present within the different renal tubular epithelial segments.
肾脏中多样的上皮细胞类型被分隔成肾单位节段和集合管，以便赋予每个肾小管节段独特的功能。每个肾单位节段中表达的独特膜通道和受体突出了上皮细胞类型的丰富多样性。我们之前的工作确定了Myh9和Myh10在哺乳动物内吞作用中的关键作用。在这里，我们研究了由Myh9、Myh10和Myh14编码的非肌肉肌球蛋白2（NM2）重链在小鼠肾脏中的表达模式，因为这些基因可能赋予独特的肾单位节段特异性膜转运特性。有趣的是，我们发现肾小管的每个节段主要仅表达三种NM2异构体中的两种，具有异构体特异性的亚细胞定位，并且在一个肾单位节段内表达水平不同。此外，我们确定Myh14仅限于闰细胞，而Myh10仅限于集合管和连接段内的主细胞。我们推测，NM2蛋白独特的表达模式可能反映了不同肾小管上皮节段中存在的细胞内运输机制的多样性。
