College of Life and Environmental Science, Wenzhou University, Wenzhou, Zhejiang 325035. China.
Curr Mol Med. 2017;17(5):350-358. doi: 10.2174/1566524018666171205115516.
Aging can result in a progressive decline in cellular protection and physiological function. The physiological status and microbiota of the intestine are intensively linked to the aging process. Numerous studies have suggested that Sargassum fusiforme polysaccharides possess antioxidant and antiaging activities. However, it remains unclear as to whether S. fusiforme polysaccharides can prevent the insult of oxidant and aging through modulating the physiological status and microbiota of the intestine.
To assess the cytoprotective effect of S. fusiforme polysaccharides (SFPS) in the small intestine of mice embarking on the aging process, we compared the expressions of Nrf2 and several representative markers (SOD-2, CAT, NQO1 and HO-1) of the Nrf2/ARE signaling pathway in the small intestine of male ICR mice fed with a control diet with those of mice fed with a SFPS-containing diet. In addition, the overall compositional changes in the small intestine microbiota were determined by 16s rDNA sequencing followed by alpha and beta diversity analyses.
The results showed that dietary intake of SFPS could ameliorate the declined cytoprotective capacity of the small intestine by upregulating the Nrf2/ARE signaling pathway. Moreover, SFPS could partially rejuvenate the overall status of the small intestine microbiota.
The results suggested that dietary SFPS may promote the health condition of the small intestine, consequently leading to a healthy aging.
衰老会导致细胞保护和生理功能逐渐下降。肠道的生理状态和微生物群与衰老过程密切相关。许多研究表明，羊栖菜多糖具有抗氧化和抗衰老作用。然而，目前尚不清楚羊栖菜多糖是否可以通过调节肠道的生理状态和微生物群来防止氧化剂和衰老的侵害。
为了评估羊栖菜多糖（SFPS）在开始衰老过程的小鼠小肠中的细胞保护作用，我们比较了对照组和 SFPS 饮食组雄性 ICR 小鼠小肠中 Nrf2 及其 Nrf2/ARE 信号通路的几个代表性标志物（SOD-2、CAT、NQO1 和 HO-1）的表达。此外，通过 16s rDNA 测序及 alpha 和 beta 多样性分析，确定了小肠微生物群的整体组成变化。
结果表明，SFPS 的饮食摄入可以通过上调 Nrf2/ARE 信号通路来改善小肠保护能力的下降。此外，SFPS 可以部分恢复小肠微生物群的整体状态。
这些结果表明，SFPS 的饮食摄入可能会促进小肠的健康状况，从而导致健康的衰老。
