Scarpelli Beatriz Brandão, Punhagui Marília Franco, Hoeppner Márcio Grama, Almeida Ricardo Sergio Couto de, Juliani Felipe Augusto, Guiraldo Ricardo Danil, Berger Sandrine Bittencourt
Department of Paediatric Dentistry, UEL - Universidade Estadual de Londrina, Londrina, PR, Brazil.
Department of Restorative Dentistry, UNOPAR - Universidade do Norte do Paraná, Londrina, PR, Brazil.
Braz Dent J. 2017 Nov-Dec;28(6):738-743. doi: 10.1590/0103-6440201701365.
Cariostatic treatment has been shown to successfully arrest caries. However, it blackens the carious tooth structure. This study evaluated the effects of an experimental cariostatic agent with silver nanoparticles (Ag-Nano) using microhardness (MH) and microbiological tests. The cariostatic agents tested were: Saforide®, Cariestop®, Ancarie® and Ag-Nano. Sixty-six samples from deciduous enamel were submitted to initial (after pH cycling to obtain initial caries-like lesion) and final (after cariostatic application) MH testing and %MH values were calculated. After longitudinal sectioning, internal (I) MH was evaluated. Strains of Streptococcus mutans, Escherichia coli, and Enterococcus faecalis in brain-heart infusion culture were treated with the cariostatic agents. Agar diffusion tests (ADTs) were performed and minimum inhibitory concentrations were determined. The statistical tests used were: Kruskal-Wallis and Dunn (%MD; ADT; MIC) and ANOVA followed by Tukey's test (I-MH) (p<0.05). The %MH of Saforide® was significantly greater than that of Ag-Nano (p<0.05). Internal MH showed progressive improvement in the enamel remineralization for all cariostatic tested. In ADTs showed greater inhibition of S. mutans, E. faecalis, and E. coli by Saforide® than by Ancarie® and Ag-Nano. Ag-Nano was able to inhibit 100% microorganism growth at a lower concentration than required for the other agents. It was concluded that Ag-Nano treatment promoted remineralization of deciduous tooth enamel with initial caries-like lesion and bactericidal activity.
抑龋治疗已被证明能成功阻止龋齿发展。然而，它会使龋坏的牙齿结构变黑。本研究使用显微硬度（MH）和微生物学测试评估了一种含银纳米颗粒（Ag-Nano）的实验性抑龋剂的效果。所测试的抑龋剂有：Saforide®、Cariestop®、Ancarie®和Ag-Nano。对66个乳牙釉质样本进行初始（pH循环后以获得初始龋样病变）和最终（抑龋剂应用后）的MH测试，并计算%MH值。纵向切片后，评估内部（I）MH。用抑龋剂处理脑心浸液培养基中的变形链球菌、大肠杆菌和粪肠球菌菌株。进行琼脂扩散试验（ADT）并确定最低抑菌浓度。所使用的统计测试有：Kruskal-Wallis和Dunn检验（%MD；ADT；MIC）以及方差分析后进行Tukey检验（I-MH）（p<0.05）。Saforide®的%MH显著高于Ag-Nano（p<0.05）。内部MH显示，所有测试的抑龋剂在釉质再矿化方面都有逐步改善。在ADT中，Saforide®对变形链球菌、粪肠球菌和大肠杆菌的抑制作用大于Ancarie®和Ag-Nano。Ag-Nano能够在比其他药剂所需浓度更低的情况下抑制100%的微生物生长。得出的结论是，Ag-Nano处理促进了具有初始龋样病变的乳牙釉质的再矿化以及杀菌活性。
