Ximenes Marcos, Cardoso Mariane, Astorga Fernando, Arnold Roland, Pimenta Luiz André, Viera Ricardo de Sousa
Universidade Federal de Santa Catarina - UFSC, Dental School, Department of Pediatric Dentistry, Florianópolis, SC, Brazil.
University of Colorado - UC, Dental School of Dental Medicine, Department of Restorative Dentistry, Denver, CO, USA.
Braz Oral Res. 2017 Jan 5;31:e2. doi: 10.1590/1807-3107BOR-2017.vol31.0002.
An early childhood carie (ECC) is an extremely destructive form of tooth decay. The aim of this study was to investigate the action of ozone (O3), and the association of sodium fluoride (NaF) with chlorhexidine (CHX) on bacteria related to ECC. Overnight culture of the bacteria was performed. On exponential phase the suspension was adjusted (101-108 CFU/mL). A drop (10μL) of each concentration of bacteria was applied on sheep blood agar plates and treated with O3 (2, 20, 200, and 2,000 ppm); after 18 hours, recovery analysis of CFU verified the reduction of bacterial activity. For NaF-CHX, sterile 96-well plates were prepared and divided into groups: G1 (150 µL TSB); G2 (20 µL of bacteria + 25 µL CHX + 25 µL NaF); and G3 (150 µL TSB + 20 µL of bacteria + 50 µL water). The plates were verified by analysis of the optical density (0, 12, 14, 16, and 18 hours). The data from O3 test were submitted to ANOVA and Tukey's test (p < 0.05). For the data from NaF-CHX, the ANOVA 2-way and Bonferroni's test (p < 0.05) were used. The number of CFU/mL showed death > 3log10 (99.9%) for all bacteria (ozone ≥ 20ppm), while the combination of NaF-CHX was more effective (p < 0.001) compared to each substance tested alone and the control group. The antimicrobial agents tested were able to inhibit all bacteria tested; O3 seemed to be a good alternative for controlling progression of carious lesions, while the association of NaF-CHX showed to be a good antimicrobial with easy and inexpensive application.
幼儿龋（ECC）是一种极具破坏性的龋齿形式。本研究的目的是调查臭氧（O₃）的作用，以及氟化钠（NaF）与洗必泰（CHX）联合使用对与ECC相关细菌的影响。对细菌进行过夜培养。在指数生长期将菌悬液调整至（10¹ - 10⁸ CFU/mL）。将每种浓度的细菌滴（10μL）接种于绵羊血琼脂平板上，并用O₃（2、20、200和2000 ppm）处理；18小时后，通过CFU恢复分析验证细菌活性的降低。对于NaF - CHX，制备无菌96孔板并分为几组：G1（150 μL胰酪大豆胨肉汤）；G2（20 μL细菌 + 25 μL CHX + 25 μL NaF）；G3（150 μL胰酪大豆胨肉汤 + 20 μL细菌 + 50 μL水）。通过分析光密度（0、12、14、16和18小时）对平板进行验证。O₃试验的数据进行方差分析和Tukey检验（p < 0.05）。对于NaF - CHX的数据，使用双向方差分析和Bonferroni检验（p < 0.05）。所有细菌（臭氧≥20 ppm）的CFU/mL数量显示死亡> 3log₁₀（99.9%），而与单独测试的每种物质和对照组相比，NaF - CHX联合使用更有效（p < 0.001）。所测试的抗菌剂能够抑制所有测试细菌；O₃似乎是控制龋损进展的良好替代品，而NaF - CHX联合使用显示是一种易于应用且成本低廉的良好抗菌剂。
