Köhler B, Bratthall D
J Clin Microbiol. 1979 May;9(5):584-8. doi: 10.1128/jcm.9.5.584-588.1979.
A method was developed to facilitate the estimation of Streptococcus mutans levels in saliva. Saliva-contaminated wodden spatulas were pressed directly against an elevated agar plate containing a selective medium. The results were compared with the number of S. mutans per 1 ml of paraffin-stimulated saliva. It was shown that the spatula method gave a good estimation of the level of S. mutans infection. The incubation was also made in expired air instead of 95% N2-5% CO2. The outgrowth was in good agreement with that after conventional incubation. The method is useful in epidemiological studies or in selecting persons at a high caries risk, and when ordinary saliva sampling cannot be done, for example in small children. Compared with conventional saliva sampling, this method requires less time and material at sampling as well as at the laboratory.
开发了一种便于估计唾液中变形链球菌水平的方法。将受唾液污染的木质刮勺直接压在含有选择性培养基的抬高琼脂平板上。将结果与每1毫升石蜡刺激唾液中的变形链球菌数量进行比较。结果表明，刮勺法能很好地估计变形链球菌感染水平。培养也是在呼出气体中进行，而不是在95% N₂ - 5% CO₂中。其生长情况与传统培养后的生长情况高度一致。该方法在流行病学研究或选择高龋风险人群时很有用，并且在无法进行常规唾液采样的情况下（例如在幼儿中）也适用。与传统唾液采样相比，该方法在采样和实验室操作时所需的时间和材料都更少。
