Wang Siqi, Wang Hengwei, Lv Jiyang, Deng Zixin, Cheng Hairong
State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University , Shanghai 200240, China.
Innovation and Application Institute (IAI), Zhejiang Ocean University , Zhoushan 316022, China.
J Agric Food Chem. 2017 Dec 20;65(50):11020-11028. doi: 10.1021/acs.jafc.7b04112. Epub 2017 Dec 12.
Erythritol, a natural sugar alcohol, is produced industrially by fermentation and crystallization, but this process leaves a large amount of waste erythritol mother liquor (WEML) which contains more than 200 g/L erythritol as well as other polyol byproducts. These impurities make it very difficult to crystallize more erythritol. In our study, an efficient process for the recovery of erythritol from the WEML is described. The polyol impurities were first identified by high-performance liquid chromatography and gas chromatography-mass spectrometry, and a yeast strain Candida maltosa CGMCC 7323 was then isolated to metabolize those impurities to purify erythritol. Our results demonstrated that the process could remarkably improve the purity of erythritol and thus make the subsequent crystallization easier. This newly developed strategy is expected to have advantages in WEML treatment and provide helpful information with regard to green cell factories and zero-waste processing.
赤藓糖醇是一种天然糖醇，通过发酵和结晶进行工业化生产，但该过程会产生大量废赤藓糖醇母液（WEML），其中含有超过200 g/L的赤藓糖醇以及其他多元醇副产物。这些杂质使得进一步结晶更多赤藓糖醇变得非常困难。在我们的研究中，描述了一种从WEML中回收赤藓糖醇的有效方法。首先通过高效液相色谱和气相色谱 - 质谱法鉴定多元醇杂质，然后分离出一株麦芽糖假丝酵母CGMCC 7323酵母菌株，使其代谢这些杂质以纯化赤藓糖醇。我们的结果表明，该方法可以显著提高赤藓糖醇的纯度，从而使后续结晶更容易。这种新开发的策略有望在WEML处理方面具有优势，并为绿色细胞工厂和零废物处理提供有用信息。
