Laboratory of Biochemistry and Protein Chemistry, Department of Cell Biology, University of Brasilia, 70910-900, DF, Brazil.
Laboratory of Biochemistry and Microorganisms, Biological Sciences and Health Center, Federal University of Mato Grosso do Sul, Campo Grande, 79070-900, MS, Brazil.
Enzyme Microb Technol. 2018 Feb;109:1-10. doi: 10.1016/j.enzmictec.2017.08.007. Epub 2017 Aug 24.
Filamentous fungal secretomes comprise highly dynamic sets of proteins, including multiple carbohydrate active enzymes (CAZymes) which are able to hydrolyze plant biomass polysaccharides into products of biotechnological interest such as fermentable sugars. In recent years, proteomics has been used to identify and quantify enzymatic and non-enzymatic polypeptides present in secretomes of several fungi species. The resulting data have widened the scientific understanding of the way filamentous fungi perform biomass degradation and offered novel perspectives for biotechnological applications. The present review discusses proteomics approaches that have been applied to the study of fungal secretomes, focusing on two of the most studied filamentous fungi genera: Trichoderma and Aspergillus.
丝状真菌的分泌组包含高度动态的蛋白质组，包括多种碳水化合物活性酶（CAZymes），它们能够将植物生物质多糖水解成具有生物技术意义的产物，如可发酵糖。近年来，蛋白质组学已被用于鉴定和定量几种真菌物种分泌组中存在的酶和非酶多肽。由此产生的数据拓宽了我们对丝状真菌进行生物质降解的方式的科学认识，并为生物技术应用提供了新的视角。本综述讨论了已应用于真菌分泌组研究的蛋白质组学方法，重点介绍了研究最多的两种丝状真菌属：木霉属和曲霉属。
