INRAE, Aix Marseille Univ., Biodiversité et Biotechnologie Fongiques, UMR1163, Marseille, France; IFP Energies nouvelles, 1 et 4 avenue de Bois-Préau, 92852 Rueil-Malmaison, France.
IFP Energies nouvelles, 1 et 4 avenue de Bois-Préau, 92852 Rueil-Malmaison, France.
Biotechnol Adv. 2021 Nov 15;52:107833. doi: 10.1016/j.biotechadv.2021.107833. Epub 2021 Sep 3.
Bioeconomy is seen as a way to mitigate the carbon footprint of human activities by reducing at least part of the fossil resources-based economy. In this new paradigm of sustainable development, the use of enzymes as biocatalysts will play an increasing role to provide services and goods. In industry, most of multicomponent enzyme cocktails are of fungal origin. Filamentous fungi secrete complex enzyme sets called "secretomes" that can be utilized as enzyme cocktails to valorize different types of bioresources. In this review, we highlight recent advances in the study of fungal secretomes using improved computational and experimental secretomics methods, the progress in the understanding of industrially important fungi, and the discovery of new enzymatic mechanisms and interplays to degrade renewable resources rich in polysaccharides (e.g. cellulose). We review current biotechnological applications focusing on the benefits and challenges of fungal secretomes for industrial applications with some examples of commercial cocktails of fungal origin containing carbohydrate-active enzymes (CAZymes) and we discuss future trends.
生物经济被视为一种通过减少至少部分基于化石资源的经济来减轻人类活动碳足迹的方法。在这种新的可持续发展模式下，酶作为生物催化剂的使用将发挥越来越重要的作用，为提供服务和商品。在工业中，大多数多组分酶混合物来自真菌。丝状真菌分泌复杂的酶组，称为“分泌组”，可被用作酶混合物来提高不同类型生物资源的价值。在这篇综述中，我们强调了使用改进的计算和实验分泌组学方法研究真菌分泌组的最新进展，以及对工业上重要真菌的理解的进展，以及发现新的酶机制和相互作用来降解富含多糖（如纤维素）的可再生资源。我们回顾了当前的生物技术应用，重点讨论了真菌分泌组在工业应用中的优势和挑战，并举例说明了一些含有碳水化合物活性酶 (CAZymes) 的真菌来源的商业混合物，并讨论了未来的趋势。
