氰基吲哚荧光寿命的溶剂依赖性

Hilaire Mary Rose, Mukherjee Debopreeti, Troxler Thomas, Gai Feng

Department of Chemistry, University of Pennsylvania, 231 South 34 Street, Philadelphia, PA 19104, USA.

Ultrafast Optical Processes Laboratory, University of Pennsylvania, 231 South 34 Street, Philadelphia, PA 19104, USA.

Chem Phys Lett. 2017 Oct 1;685:133-138. doi: 10.1016/j.cplett.2017.07.038. Epub 2017 Jul 18.

Several cyanotryptophans have been shown to be useful biological fluorophores. However, how their fluorescence lifetimes vary with solvent has not been examined. In this regard, herein we measure the fluorescence decay kinetics as well as the absorption and emission spectra of six cyanoindoles in different solvents. In particular, we find, among other results, that only 4-cyanoindole affords a long fluorescence lifetime and hence high quantum yield in HO. Therefore, our measurements provide not only a guide for choosing which cyanotryptophan to use in practice but also data for computational modeling of the substitution effect on the electronic transitions of indole.

几种氰基色氨酸已被证明是有用的生物荧光团。然而，它们的荧光寿命如何随溶剂变化尚未得到研究。在这方面，我们在此测量了六种氰基吲哚在不同溶剂中的荧光衰减动力学以及吸收光谱和发射光谱。特别是，我们发现，除其他结果外，只有4-氰基吲哚在水中具有较长的荧光寿命，因此具有较高的量子产率。因此，我们的测量不仅为实际应用中选择使用哪种氰基色氨酸提供了指导，也为吲哚电子跃迁取代效应的计算建模提供了数据。