Zaidi M, Moonga B, Moss D W, MacIntyre I
Department of Chemical Pathology, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Biochem Biophys Res Commun. 1989 Feb 28;159(1):68-71. doi: 10.1016/0006-291x(89)92405-4.
Osteoclastic acid phosphatase is a member of a widely-distributed class of iron-containing proteins with acid phosphatase activity. Antibodies raised against one member of this class cross-react with other members from the same or different species, but not with acid phosphatase isoenzymes of different types. When antibodies to one such protein, porcine uteroferrin, are added to medium in which rat osteoclasts are incubated on devitalised cortical bone, both bone resorption and acid phosphatase activity are markedly inhibited. Furthermore, addition of molybdate (an inhibitor of this class of acid phosphatases) also inhibits both bone resorption and enzyme activity. These observations strongly suggest a functional role for osteoclastic acid phosphatase in bone resorption.
破骨细胞酸性磷酸酶是一类广泛分布的含铁蛋白质且具有酸性磷酸酶活性。针对该类中一个成员产生的抗体可与来自相同或不同物种的其他成员发生交叉反应，但不与不同类型的酸性磷酸酶同工酶发生反应。当将针对一种此类蛋白质（猪子宫运铁蛋白）的抗体添加到培养基中时，在该培养基中大鼠破骨细胞在失活的皮质骨上进行孵育，骨吸收和酸性磷酸酶活性均受到显著抑制。此外，添加钼酸盐（这类酸性磷酸酶的一种抑制剂）也会抑制骨吸收和酶活性。这些观察结果有力地表明破骨细胞酸性磷酸酶在骨吸收中具有功能性作用。
