牙髓干细胞移植可改善长期糖尿病多发性神经病，并改善神经形态计量评估。

Transplantation of dental pulp stem cells improves long-term diabetic polyneuropathy together with improvement of nerve morphometrical evaluation.

机构信息

Department of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, 2-11 Suemori-dori, Chikusa-ku, Nagoya, Aichi, 464-8651, Japan.

Department of Internal Medicine, School of Dentistry, Aichi Gakuin University, 2-11 Suemori-dori, Chikusa-ku, Nagoya, Aichi, 464-8651, Japan.

出版信息

Stem Cell Res Ther. 2017 Dec 13;8(1):279. doi: 10.1186/s13287-017-0729-5.

BACKGROUND

Although previous reports have revealed the therapeutic potential of stem cell transplantation in diabetic polyneuropathy, the effects of cell transplantation on long-term diabetic polyneuropathy have not been investigated. In this study, we investigated whether the transplantation of dental pulp stem cells (DPSCs) ameliorated long-term diabetic polyneuropathy in streptozotocin (STZ)-induced diabetic rats.

METHODS

Forty-eight weeks after STZ injection, we transplanted DPSCs into the unilateral hindlimb skeletal muscles. Four weeks after DPSC transplantation (i.e., 52 weeks after STZ injection) the effects of DPSC transplantation on diabetic polyneuropathy were assessed.

RESULTS

STZ-induced diabetic rats showed significant reductions in the sciatic motor/sensory nerve conduction velocity, increases in the current perception threshold, and decreases in capillary density in skeletal muscles and intra-epidermal nerve fiber density compared with normal rats, all of which were ameliorated by DPSC transplantation. Furthermore, sural nerve morphometrical analysis revealed that the transplantation of DPSCs significantly increased the myelin thickness and area. DPSC-conditioned media promoted the neurite outgrowth of dorsal root ganglion neurons and increased the viability and myelin-related protein expression of Schwann cells.

CONCLUSIONS

These results indicated that the transplantation of DPSCs contributed to the neurophysiological and neuropathological recovery from a long duration of diabetic polyneuropathy.

背景

虽然先前的报告揭示了干细胞移植在糖尿病多发性神经病中的治疗潜力，但细胞移植对长期糖尿病多发性神经病的影响尚未得到研究。在这项研究中，我们研究了牙髓干细胞（DPSCs）移植是否改善了链脲佐菌素（STZ）诱导的糖尿病大鼠的长期糖尿病多发性神经病。

方法

在 STZ 注射后 48 周，我们将 DPSCs 移植到单侧后肢骨骼肌中。在 DPSC 移植后 4 周（即 STZ 注射后 52 周）评估 DPSC 移植对糖尿病多发性神经病的影响。

结果

与正常大鼠相比，STZ 诱导的糖尿病大鼠的坐骨运动/感觉神经传导速度显著降低，电流感知阈值升高，骨骼肌和表皮内神经纤维密度降低，所有这些都通过 DPSC 移植得到改善。此外，腓肠神经形态计量学分析显示，DPSC 移植显著增加了髓鞘厚度和面积。DPSC 条件培养基促进背根神经节神经元的神经突生长，并增加施万细胞的活力和髓鞘相关蛋白表达。