Xing Liping, Di Zhaocan, Yang Wenwu, Liu Jiaqian, Li Meina, Wang Xiaojuan, Cui Chaofan, Wang Xiaoyun, Wang Xiue, Zhang Ruiqi, Xiao Jin, Cao Aizhong
National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, China.
Front Plant Sci. 2017 Nov 29;8:1948. doi: 10.3389/fpls.2017.01948. eCollection 2017.
The APETALA 2/Ethylene-responsive element binding factor (AP2/ERF) transcription factor gene family is widely involved in the biotic and abiotic stress regulation. (VV, 2 = 14), a wild species of wheat, is a potential gene pool for wheat improvement. confers high resistance to several wheat diseases and high tolerance to some abiotic stress. In this study, , an ethylene-responsive element-binding factor gene of the AP2/ERF transcription factor gene family from wild , was cloned and characterized. Sequence and phylogenetic analysis showed that is a deduced B2 type gene. was first identified as a f. sp. ( up-regulated gene, and later found to be induced by drought, salt and cold stresses. In responses to hormones, was up-regulated by ethylene and abscisic acid, but down-regulated by salicylic acid and jasmonic acid. Over expression of in wheat could improve resistance to powdery mildew, salt and drought stress. Chlorophyll content, malondialdehyde content, superoxide dismutase and peroxidase activity were significantly differences between the recipient Yangmai158 and the transgenic plants following salt treatment. Furthermore, the expression levels of some stress responsive genes were differences after drought or salt treatments. Although was activated by the constitutive promoter, the agronomic traits, including flowering time, plant height, effective tiller number, spikelet number per spike and grain size, did not changed significantly. is a valuable gene for wheat improvement by genetic engineering.
APETALA 2/乙烯响应元件结合因子（AP2/ERF）转录因子基因家族广泛参与生物和非生物胁迫调控。野生二粒小麦（Triticum dicoccoides，2n = 4x = 28，AABB）是小麦改良的潜在基因库，对多种小麦病害具有高抗性，对一些非生物胁迫具有高耐受性。在本研究中，从小麦野生近缘种二粒小麦中克隆并鉴定了AP2/ERF转录因子基因家族的一个乙烯响应元件结合因子基因TaERF3。序列和系统发育分析表明，TaERF3是一个推导的B2型ERF基因。TaERF3首先被鉴定为条锈菌（Puccinia striiformis f. sp. tritici）诱导上调基因，后来发现其受干旱、盐和冷胁迫诱导。在激素响应方面，TaERF3受乙烯和脱落酸上调，但受水杨酸和茉莉酸下调。TaERF3在小麦中的过表达可提高对白粉病、盐和干旱胁迫的抗性。盐处理后，受体扬麦158和转基因植株之间的叶绿素含量、丙二醛含量、超氧化物歧化酶和过氧化物酶活性存在显著差异。此外，干旱或盐处理后一些胁迫响应基因的表达水平也存在差异。虽然TaERF3由组成型启动子激活，但其农艺性状，包括开花时间、株高、有效分蘖数、每穗小穗数和粒大小，没有显著变化。TaERF3是通过基因工程改良小麦的一个有价值的基因。
