Teng C M, Kuo S C, Ko F N, Lee J C, Lee L G, Chen S C, Huang T F
Pharmacological Institute, College of Medicine, National Taiwan University, Taipei.
Biochim Biophys Acta. 1989 Mar 24;990(3):315-20. doi: 10.1016/s0304-4165(89)80051-0.
The antiplatelet effect of panaxynol isolated from the diethyl ether layer was compared with those of ginsenosides from the butanol layer of Panax ginseng. Panaxynol (0.1 mg/ml) inhibited markedly the aggregation of washed platelets induced by collagen, arachidonic acid, ADP, ionophore A23187, PAF and thrombin while ginsenosides had no significant effect on the aggregation but ginsenoside Ro (1 mg/ml) inhibited the ATP release of platelets. Less inhibitory effect of panaxynol was observed in the aggregation of platelet-rich plasma. Thromboxane B2 formation of platelets was inhibited by panaxynol but not by ginsenosides. The antiplatelet effect of panaxynol was dependent on the incubation time and the aggregability of platelets inhibited by panaxynol could not easily be recovered after washing the platelets. In human platelet-rich plasma, panaxynol prevented secondary aggregation and completely blocked ATP release from platelets induced by epinephrine and ADP. Both panaxynol and ginsenoside Rg2 inhibited the rise of intracellular calcium caused by collagen. It is concluded that panaxynol is the most potent antiplatelet agent in ginseng and its mechanism of action is chiefly due to the inhibition of thromboxane formation.
将从人参乙醚层分离得到的人参炔醇的抗血小板作用与人参正丁醇层的人参皂苷的抗血小板作用进行了比较。人参炔醇（0.1毫克/毫升）显著抑制由胶原、花生四烯酸、二磷酸腺苷、离子载体A23187、血小板活化因子和凝血酶诱导的洗涤血小板的聚集，而人参皂苷对血小板聚集无显著影响，但人参皂苷Ro（1毫克/毫升）抑制血小板的ATP释放。在富含血小板血浆的聚集过程中观察到人参炔醇的抑制作用较小。人参炔醇抑制血小板血栓素B2的形成，而人参皂苷则无此作用。人参炔醇的抗血小板作用取决于孵育时间，且经洗涤后，人参炔醇抑制的血小板聚集能力不易恢复。在人富含血小板血浆中，人参炔醇可防止继发性聚集，并完全阻断由肾上腺素和二磷酸腺苷诱导的血小板ATP释放。人参炔醇和人参皂苷Rg2均抑制由胶原引起的细胞内钙升高。得出的结论是，人参炔醇是人参中最有效的抗血小板剂，其作用机制主要是由于抑制血栓素的形成。
