Regional Medical Research Centre (ICMR), Chandrasekharpur, India.
KIIT School of Biotechnology, Kalinga Institute of Industrial Technology, Bhubaneswar, India.
Pest Manag Sci. 2018 Jun;74(6):1431-1440. doi: 10.1002/ps.4827. Epub 2018 Feb 26.
Aedes albopictus is currently the most invasive mosquito species in the world. Keeping in view the wide emergence of insecticide resistance, it is imperative to focus on the current susceptibility status for various insecticides in Ae. albopictus. This study focused on understanding the insecticide resistance mechanism of Ae. albopictus collected from dengue-endemic districts of Odisha.
Insecticide resistance was evaluated by using standardized bioassay kits (WHO) and biochemical analysis. Larval bioassays revealed the highest level of resistance from Jaipur (JP) population with a RR of 15.3 and LC of 1.177 ppm compared with an LC of 0.077 for the susceptible strain LabS. Results indicated the presence of dichlorodiphenyltrichloroethane resistance in the majority of adult populations. Elevated activity of nonspecific esterases and cytochrome P450s MFO indicated probable resistance to organophosphates and pyrethroids. Molecular screening for common insecticide target-site mutations confirmed the absence of the 'knockdown resistance' response for pyrethroid insecticide in Ae. albopictus population, suggesting its continual effectiveness as the major insecticide of significant importance in future vector-control programmes.
This is the first report of a kdr mutation in Ae. albopictus in India and highlights the need for intensive research on other unexplored target-site mutations that might also contribute to pyrethroid resistance. Effective management and sustainable use of insecticides can be implemented by understanding resistance mechanisms and development of appropriate diagnostic tools. © 2017 Society of Chemical Industry.
白纹伊蚊是目前世界上最具侵袭性的蚊子物种。鉴于杀虫剂抗性的广泛出现，必须关注白纹伊蚊对各种杀虫剂的当前敏感性状况。本研究集中于了解来自奥里萨邦登革热流行地区的白纹伊蚊的杀虫剂抗性机制。
使用标准化生物测定试剂盒（世卫组织）和生化分析来评估杀虫剂抗性。幼虫生物测定显示来自斋浦尔（JP）种群的最高抗性水平，RR 为 15.3，LC 为 1.177 ppm，而敏感株 LabS 的 LC 为 0.077 ppm。结果表明，大多数成年种群都存在滴滴涕抗性。非特异性酯酶和细胞色素 P450s MFO 的活性升高表明可能对有机磷和拟除虫菊酯类杀虫剂产生抗性。常见杀虫剂靶标基因突变的分子筛选证实了白纹伊蚊种群对拟除虫菊酯类杀虫剂的“击倒抗性”反应缺失，这表明其作为未来病媒控制计划中重要杀虫剂的持续有效性。
这是印度首次报道白纹伊蚊存在 kdr 突变，并强调需要对其他可能导致拟除虫菊酯类杀虫剂抗性的未探索靶标突变进行深入研究。通过了解抗性机制和开发适当的诊断工具，可以实施有效的管理和可持续使用杀虫剂。© 2017 化学工业协会。
