Bharati Minu, Rai Priyanka, Saha Dhiraj
Insect Biochemistry and Molecular Biology Laboratory, Department of Zoology, University of North Bengal, Raja Rammohunpur, P.O. North Bengal University, Siliguri, 734013, District - Darjeeling, West Bengal, India.
Insect Biochemistry and Molecular Biology Laboratory, Department of Zoology, University of North Bengal, Raja Rammohunpur, P.O. North Bengal University, Siliguri, 734013, District - Darjeeling, West Bengal, India.
Acta Trop. 2019 Apr;192:104-111. doi: 10.1016/j.actatropica.2019.02.007. Epub 2019 Feb 11.
Dengue is one of the most rapidly spreading infectious diseases prevalent throughout the tropical and sub-tropical regions of the world. In absence of specific medications and vaccines, the sole method of disease prevention relies on vector control mainly using insecticides. But with the advent of Insecticide resistance, worldwide vector control programs are facing failure. In this study, eleven different Ae. albopictus population from sub-Himalayan districts of West Bengal, India were investigated as per WHO protocols to find out the current status of insecticide susceptibility against DDT, permethrin and propoxur. Also the role of three insecticide detoxifying enzymes underlying observed resistance was investigated through quantitative and synergistic assays to unveil the mechanism of insecticide resistance. It was found that majority of studied populations were resistant to 4% DDT. Two populations, namely Alipurduar (APD) and Jalpaiguri (JPG) were severely resistant to 0.75% permethrin, whereas only JPG population was found to exhibit severe resistance against 0.1% propoxur. Moreover, the involvement of detoxifying enzymes was also noted in conferring resistance against DDT and Permethrin. This study indicates the inefficacy of DDT in controlling Ae. albopictus populations in the study region. This study may help in implementation of an efficient vector control and insecticide resistance management strategies.
登革热是世界热带和亚热带地区传播速度最快的传染病之一。由于缺乏特效药物和疫苗，疾病预防的唯一方法主要依靠使用杀虫剂进行病媒控制。但随着杀虫剂抗性的出现，全球病媒控制项目正面临失败。在本研究中，按照世界卫生组织的方案，对来自印度西孟加拉邦喜马拉雅山地区的11个不同白纹伊蚊种群进行了调查，以了解其对滴滴涕、氯菊酯和残杀威的杀虫剂敏感性现状。此外，还通过定量和增效试验研究了三种杀虫剂解毒酶在观察到的抗性中的作用，以揭示杀虫剂抗性的机制。结果发现，大多数研究种群对4%的滴滴涕具有抗性。两个种群，即阿里布尔杜阿尔（APD）和贾尔派古里（JPG）对0.75%的氯菊酯具有严重抗性，而只有JPG种群对0.1%的残杀威表现出严重抗性。此外，还发现解毒酶参与了对滴滴涕和氯菊酯的抗性。本研究表明滴滴涕在控制研究区域的白纹伊蚊种群方面无效。这项研究可能有助于实施有效的病媒控制和杀虫剂抗性管理策略。
