Grabska-Zielińska Sylwia, Sosik Adrianna, Małkowska Anna, Olewnik-Kruszkowska Ewa, Steinbrink Kerstin, Kleszczyński Konrad, Kaczmarek-Szczepańska Beata
Department of Physical Chemistry and Physicochemistry of Polymers, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100 Toruń, Poland.
Department of Biomaterials and Cosmetics Chemistry, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100 Toruń, Poland.
Materials (Basel). 2021 Sep 1;14(17):4993. doi: 10.3390/ma14174993.
In this work, two-component dialdehyde chitosan/hyaluronic acid scaffolds were developed and characterized. Dialdehyde chitosan was obtained by one-step synthesis with chitosan and sodium periodate. Three-dimensional scaffolds were prepared by the lyophilization method. Fourier transform infrared spectroscopy (FTIR) was used to observe the chemical structure of scaffolds and scanning electron microscopy (SEM) imaging was done to assess the microstructure of resultant materials. Thermal analysis, mechanical properties measurements, density, porosity and water content measurements were used to characterize physicochemical properties of dialdehyde chitosan/hyaluronic acid 3D materials. Additionally, human epidermal keratinocytes (NHEK), dermal fibroblasts (NHDF) and human melanoma cells (A375 and G-361) were used to evaluate cell viability in the presence of subjected scaffolds. It was found that scaffolds were characterized by a porous structure with interconnected pores. The scaffold composition has an influence on physicochemical properties, such as mechanical strength, thermal resistance, porosity and water content. There were no significant differences between cell viability proliferation of all scaffolds, and this observation was visible for all subjected cell lines.
在本研究中，制备并表征了双组分二醛壳聚糖/透明质酸支架。二醛壳聚糖通过壳聚糖与高碘酸钠一步合成法获得。采用冻干法制备三维支架。利用傅里叶变换红外光谱（FTIR）观察支架的化学结构，并通过扫描电子显微镜（SEM）成像评估所得材料的微观结构。采用热分析、力学性能测量、密度、孔隙率和含水量测量等方法对二醛壳聚糖/透明质酸三维材料的物理化学性质进行表征。此外，使用人表皮角质形成细胞（NHEK）、真皮成纤维细胞（NHDF）和人黑色素瘤细胞（A375和G-361）评估受试支架存在时的细胞活力。结果发现，支架具有相互连通的多孔结构。支架组成对物理化学性质有影响，如机械强度、耐热性、孔隙率和含水量。所有支架的细胞活力增殖之间没有显著差异，并且所有受试细胞系均观察到这一现象。
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