Nicolaus Copernicus University in Toruń, Faculty of Chemistry, Department of Biomaterials and Cosmetics Chemistry, Gagarin 7, 87-100 Toruń, Poland.
Nicolaus Copernicus University in Toruń, Faculty of Biological and Veterinary Sciences, Department of Environmental Microbiology and Biotechnology, Gagarin 9, 87-100 Toruń, Poland.
Int J Biol Macromol. 2020 Apr 15;149:290-295. doi: 10.1016/j.ijbiomac.2020.01.262. Epub 2020 Jan 28.
In this work thin film made from the mixture of collagen, hyaluronic acid and chitosan were obtained during solvent evaporation method. The mixtures of biopolymers were modified by dialdehyde starch, which was used as a crosslinking agent. The influence of crosslinking agent on the physico-chemical properties of polymeric matrices was evaluated. The interactions between functional groups of polymers were evaluated by Fourier transform infrared spectroscopy for both unmodified and modified samples. Mechanical properties of film were tested in dry condition using a mechanical testing machine. Morphology of the surface was studied by Atomic Force Microscopy and the roughness parameters were analyzed. Moreover, surface free energy and its polar and dispersive components were evaluated by contact angle measurements. It was found that the addition of dialdehyde starch modified all tested parameters of the studied films. Samples became less elastic and more resist for rupture. Moreover, samples became less rough after crosslinking process and surface free energy increased. Thin film made from the mixture of collagen, hyaluronic acid and chitosan crosslinked with dialdehyde starch can be applied in medicine and in cosmetics.
在这项工作中，通过溶剂蒸发法获得了由胶原蛋白、透明质酸和壳聚糖混合物制成的薄膜。生物聚合物的混合物通过作为交联剂的双醛淀粉进行改性。研究了交联剂对聚合物基质物理化学性质的影响。通过傅里叶变换红外光谱对未改性和改性样品的聚合物官能团之间的相互作用进行了评估。使用机械试验机在干燥条件下测试了薄膜的机械性能。通过原子力显微镜研究了表面形貌，并分析了粗糙度参数。此外，通过接触角测量评估了表面自由能及其极性和分散分量。结果发现，添加双醛淀粉改性了研究薄膜的所有测试参数。交联后，样品的弹性降低，抗破裂能力增强。此外，交联过程后样品变得不那么粗糙，表面自由能增加。用双醛淀粉交联的胶原蛋白、透明质酸和壳聚糖混合物制成的薄膜可应用于医学和化妆品领域。
