Ackermann R F, Moshé S L, Albala B J
Department of Radiological Sciences, School of Medicine, University of California, Los Angeles 90024.
Exp Neurol. 1989 Apr;104(1):73-81. doi: 10.1016/0014-4886(89)90011-3.
Sixteen-day-old albino rat pups were kindled to varying degrees of seizure severity with amygdala stimulations spaced 15 to 20 min apart. Subsequently, each rat pup was injected (ip) with 10 microCi of [2-14C]-deoxyglucose, and received several additional kindled seizures at regular intervals throughout the following 80 min, at which time it was killed and processed for deoxyglucose autoradiography. Increased seizure severity was associated with correspondingly increased deoxyglucose utilization in many rhinencephalic limbic structures. However, unlike adults, rat pups did not show discernibly increased neocortical, thalamic, or substantia nigra utilization. We postulate that the apparent confinement of seizure activity to limbic structures in pups is related to their relative lack of postictal seizure refractoriness, as well as to other indices of increased seizure susceptibility in immature animals.
对16日龄的白化病幼鼠进行杏仁核刺激，刺激间隔为15至20分钟，使其引发不同程度的癫痫发作严重程度。随后，每只幼鼠经腹腔注射10微居里的[2-14C]-脱氧葡萄糖，并在接下来的80分钟内定期接受几次额外的点燃性癫痫发作，此时将其处死并进行脱氧葡萄糖放射自显影处理。癫痫发作严重程度的增加与许多嗅脑边缘结构中脱氧葡萄糖利用率的相应增加有关。然而，与成年动物不同，幼鼠的新皮质、丘脑或黑质利用率并未明显增加。我们推测，幼鼠癫痫活动明显局限于边缘结构，这与其相对缺乏发作后癫痫不应性以及未成熟动物癫痫易感性增加的其他指标有关。
