College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University , Chengdu 610065, China.
High and New Technology Research Center, Henan Academy of Sciences , Zhengzhou 450002, China.
Biomacromolecules. 2018 Feb 12;19(2):279-287. doi: 10.1021/acs.biomac.7b01016. Epub 2018 Jan 2.
A cross-linked waterborne polyurethane (CPTMGPU) with long-term stability was developed from poly(ethylene glycol) (PEG), polyoxytetramethylene glycol (PTMG), isophorone diisocyanate (IPDI), l-lysine, and its derivative diamine consisting of gemini quaternary ammonium salt (GQAS), using ethylene glycol diglycidyl ether (EGDE) as a cross-linker. Weight loss test, X-ray photoelectron spectroscopy (XPS) measurements, and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) were performed to prove the surface structure and stability of these CPTMGPU films. Furthermore, the GQAS-bearing CPTMGPUs show repeatable contact-active antibacterial efficacy against both Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) bacteria and do not show any inhibition effect against fibroblasts in vitro. After subcutaneous implantation in rats, the CPTMGPU films manifest good biocompatibility in vivo, despite the presence of a typical foreign body reaction toward surrounding tissues and mild systematic inflammation reaction that could be eliminated after a short implantation period, as demonstrated by histology and immunohistochemistry combined with interleukin (IL)-1β, IL-4, IL-6, IL-10, and TNF-α analysis though enzyme-linked immunosorbent assay (ELISA) and real-time quantitative polymerase chain reaction (qRT-PCR). Therefore, these cross-linked waterborne polyurethanes hold great promise for antibacterial applications in vivo.
开发了一种具有长期稳定性的交联型水性聚氨酯（CPTMGPU），其由聚乙二醇（PEG）、聚四氢呋喃二醇（PTMG）、异佛尔酮二异氰酸酯（IPDI）、赖氨酸及其衍生的二胺组成，Gemini 季铵盐（GQAS），使用乙二醇二缩水甘油醚（EGDE）作为交联剂。通过重量损失测试、X 射线光电子能谱（XPS）测量和衰减全反射傅里叶变换红外光谱（ATR-FTIR）证明了这些 CPTMGPU 薄膜的表面结构和稳定性。此外，含有 GQAS 的 CPTMGPUs 对革兰氏阳性金黄色葡萄球菌（S. aureus）和革兰氏阴性大肠杆菌（E. coli）具有可重复的接触活性抗菌功效，并且在体外对成纤维细胞没有任何抑制作用。在大鼠皮下植入后，CPTMGPU 薄膜在体内表现出良好的生物相容性，尽管存在典型的异物反应和轻度的系统性炎症反应，但通过组织学和免疫组织化学结合白细胞介素（IL）-1β、IL-4、IL-6、IL-10 和 TNF-α 分析（通过酶联免疫吸附试验（ELISA）和实时定量聚合酶链反应（qRT-PCR）），在短时间的植入后可以消除这些反应。因此，这些交联型水性聚氨酯在体内抗菌应用中具有广阔的应用前景。
