Zhang Yi, Ge Tao, Li Yifan, Lu Jinlin, Du Hao, Yan Ling, Tan Hong, Li Jiehua, Yin Yansheng
Engineering Technology Research Center for Corrosion Control and Protection of Materials in Extreme Marine Environment, Guangzhou Maritime University, Guangzhou 510725, China.
College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China.
Polymers (Basel). 2023 Jan 7;15(2):317. doi: 10.3390/polym15020317.
Biofilms are known to be difficult to eradicate and control, complicating human infections and marine biofouling. In this study, self-polishing and anti-fouling waterborne polyurethane coatings synthesized from gemini quaternary ammonium salts (GQAS), polyethylene glycol (PEG), and polycaprolactone diol (PCL) demonstrate excellent antibiofilm efficacy. Their anti-fouling and anti-biofilm performance was confirmed by a culture-based method in broth media, with the biofilm formation factor against Gram-positive () and Gram-negative bacterial strains () for 2 days. The results indicate that polyurethane coatings have excellent anti-biofilm activity when the content of GQAS reached 8.5 wt% against , and 15.8 wt% against . The resulting waterborne polyurethane coatings demonstrate both hydrolytic and enzymatic degradation, and the surface erosion enzymatic degradation mechanism enables them with good self-polishing capability. The extracts cyto-toxicity of these polyurethane coatings and degradation liquids was also systematically studied; they could be degraded to non-toxic or low toxic compositions. This study shows the possibility to achieve potent self-polishing and anti-biofilm efficacy by integrating antibacterial GQAS, PEG, and PCL into waterborne polyurethane coatings.
众所周知，生物膜难以根除和控制，这使人类感染和海洋生物污损问题变得更加复杂。在本研究中，由双子季铵盐（GQAS）、聚乙二醇（PEG）和聚己内酯二醇（PCL）合成的自抛光防污水性聚氨酯涂料表现出优异的抗生物膜功效。通过肉汤培养基中基于培养的方法证实了它们的防污和抗生物膜性能，针对革兰氏阳性菌（）和革兰氏阴性菌菌株（）的生物膜形成因子作用2天。结果表明，当GQAS含量达到8.5 wt%（针对）和15.8 wt%（针对）时，聚氨酯涂料具有优异的抗生物膜活性。所得的水性聚氨酯涂料表现出水解和酶促降解，表面侵蚀酶促降解机制使其具有良好的自抛光能力。还系统研究了这些聚氨酯涂料和降解液的提取物细胞毒性；它们可以降解为无毒或低毒成分。本研究表明，通过将抗菌GQAS、PEG和PCL整合到水性聚氨酯涂料中，有可能实现强大的自抛光和抗生物膜功效。
