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马来西亚雪兰莪州牛及牛肉中四环素的流行情况及耐药性

Prevalence and Antibiotic Resistance against Tetracycline in and in Cattle and Beef Meat from Selangor, Malaysia.

作者信息

Premarathne Jayasekara M K J K, Anuar Aimi S, Thung Tze Young, Satharasinghe Dilan A, Jambari Nuzul Noorahya, Abdul-Mutalib Noor-Azira, Huat John Tang Yew, Basri Dayang F, Rukayadi Yaya, Nakaguchi Yoshitsugu, Nishibuchi Mitsuaki, Radu Son

机构信息

Faculty of Food Science and Technology, Food Safety Research Center, Universiti Putra Malaysia, Seri Kembangan, Malaysia.

Department of Livestock and Avian Science, Faculty of Livestock, Fisheries and Nutrition, Wayamba University of Sri Lanka, Kuliyapitiya, Sri Lanka.

出版信息

Front Microbiol. 2017 Dec 4;8:2254. doi: 10.3389/fmicb.2017.02254. eCollection 2017.

Abstract

is a major foodborne pathogen frequently associated with human bacterial gastroenteritis in the world. This study was conducted to determine the prevalence and antibiotic resistance of spp. in the beef food system in Malaysia. A total of 340 samples consisting of cattle feces ( = 100), beef ( = 120) from wet markets and beef ( = 120) from hypermarkets were analyzed for spp. The overall prevalence of was 17.4%, consisting of 33% in cattle fecal samples, 14.2% in raw beef from wet market and 7.5% in raw beef from the hypermarket. The multiplex-polymerase chain reaction (PCR) identified 55% of the strains as , 26% as , and 19% as other spp. A high percentage of spp. were resistant to tetracycline (76.9%) and ampicillin (69.2%), whilst low resistance was exhibited to chloramphenicol (7.6%). The MAR Index of isolates from this study ranged from 0.09 to 0.73. The present study indicates the potential public health risk associated with the beef food system, hence stringent surveillance, regulatory measures, and appropriate interventions are required to minimize contamination and prudent antibiotic usage that can ensure consumer safety.

摘要

是一种主要的食源性病原体,在世界范围内经常与人类细菌性肠胃炎相关。本研究旨在确定马来西亚牛肉食品系统中该菌的流行情况和抗生素耐药性。共分析了340份样本,包括牛粪便( = 100)、来自湿货市场的牛肉( = 120)和来自超市的牛肉( = 120)中的该菌。该菌的总体流行率为17.4%,其中牛粪便样本中为33%,湿货市场生牛肉中为14.2%,超市生牛肉中为7.5%。多重聚合酶链反应(PCR)鉴定出55%的菌株为该菌,26%为该菌,19%为其他该菌属。该菌属的大部分菌株对四环素(76.9%)和氨苄青霉素(69.2%)耐药,而对氯霉素的耐药性较低(7.6%)。本研究中该菌分离株的MAR指数范围为0.09至0.73。本研究表明牛肉食品系统存在潜在的公共卫生风险,因此需要严格的监测、监管措施和适当的干预措施,以尽量减少该菌污染并谨慎使用抗生素,从而确保消费者安全。

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