Maktabi Siavash, Ghorbanpoor Masoud, Hossaini Masomeh, Motavalibashi Amirabbas
Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Vet Res Forum. 2019 Winter;10(1):37-42. doi: 10.30466/vrf.2019.34310. Epub 2019 Mar 15.
and are the main causes of gastrointestinal diseases in humans even in industrialized countries affecting public health. The aim of the current study was to evaluate the occurrence and antibiotic resistance of and in chicken meat, beef, mutton and water buffalo meat slaughtered in Ahvaz city, Iran. A total of 380 samples including chicken meat from industrial abattoirs (n = 150), chicken meat from traditional abattoirs (n = 50), fresh packed chicken meat from local markets (n = 30) and beef, mutton and water buffalo meat from industrial abattoirs (50 samples for each meat) in Ahvaz,were collected and tested for the presence of spp. The procedure was one-step enrichment in Preston enrichment broth followed by plating on supplemented blood agar for 24 hr under microaerophilic conditions at 42 ˚C. Suspected colonies were tested by polymerase chain reaction assay and susceptibility of the confirmed isolates to various antibiotics was investigated by the Kirby-Bauer disk diffusion method. Overall, 32 samples (8.40%) were contaminated with spp. Mutton was the most contaminated meat (24%), while fresh packed chicken meat were not contaminated. Among the 32 isolates, 40.60%, 34.40%, 21.90%, and 15.60% were resistant to tetracycline, ciprofloxacin, ampicillin, and streptomycin, respectively. Moreover, a high number of multi-antibiotic resistant spp. was determined. Since foods of animal origin are the most sources of Campylobacter infection, the presence of resistant strains to antibiotics is a potential risk to public health.
即便在工业化国家，[某些病菌名称未给出]也是影响公众健康的人类胃肠道疾病的主要病因。本研究的目的是评估在伊朗阿瓦士市屠宰的鸡肉、牛肉、羊肉和水牛肉中[某些病菌名称未给出]的发生情况及抗生素耐药性。总共收集了380份样本，包括来自工业屠宰场的鸡肉（n = 150）、来自传统屠宰场的鸡肉（n = 50）、当地市场的新鲜包装鸡肉（n = 30）以及来自阿瓦士工业屠宰场的牛肉、羊肉和水牛肉（每种肉50份样本），并检测其中[某些病菌名称未给出]的存在情况。检测程序是先在普雷斯顿增菌肉汤中进行一步增菌，然后在补充血琼脂上于42℃微需氧条件下培养24小时。对疑似菌落进行聚合酶链反应检测，并通过 Kirby - Bauer 纸片扩散法研究确诊分离株对各种抗生素的敏感性。总体而言，32份样本（8.40%）被[某些病菌名称未给出]污染。羊肉是污染最严重的肉类（24%），而新鲜包装鸡肉未被污染。在这32株分离株中，分别有40.60%、34.40%、21.90%和15.60%对四环素、环丙沙星、氨苄青霉素和链霉素耐药。此外，还检测到大量多重耐药的[某些病菌名称未给出]。由于动物源性食品是弯曲杆菌感染的主要来源，抗生素耐药菌株的存在对公众健康构成潜在风险。
