OSIsoft, 1600 Alvarado St, San Leandro, CA, 94577, USA.
Biodesign Center for Immunotherapy, Vaccines, and Virotherapy, The Biodesign Institute, Arizona State University, 1001 S. McAllister Ave, Tempe, AZ, 85287, USA.
BMC Biotechnol. 2017 Dec 19;17(1):89. doi: 10.1186/s12896-017-0408-8.
Precise spatial positioning and isolation of mammalian cells is a critical component of many single cell experimental methods and biological engineering applications. Although a variety of cell patterning methods have been demonstrated, many of these methods subject cells to high stress environments, discriminate against certain phenotypes, or are a challenge to implement. Here, we demonstrate a rapid, simple, indiscriminate, and minimally perturbing cell patterning method using a laser fabricated polymer stencil. The stencil fabrication process requires no stencil-substrate alignment, and is readily adaptable to various substrate geometries and experiments.
精确的空间定位和哺乳动物细胞的隔离是许多单细胞实验方法和生物工程应用的关键组成部分。虽然已经证明了多种细胞图案化方法，但其中许多方法会使细胞处于高压力环境，歧视某些表型，或者难以实施。在这里，我们展示了一种使用激光制造的聚合物模板的快速、简单、无差别和最小干扰的细胞图案化方法。模板制造过程不需要模板-基底对准，并且很容易适应各种基底几何形状和实验。
