流式病毒测量技术作为一种研究病毒的工具。
Flow virometry as a tool to study viruses.
机构信息
Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853, USA.
Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853, USA.
出版信息
Methods. 2018 Feb 1;134-135:87-97. doi: 10.1016/j.ymeth.2017.12.011. Epub 2017 Dec 16.
Abstract
In the last few decades, flow cytometry has redefined the field of biology, exponentially enhancing our understanding of cells, immunology, and microbiology. Flow cytometry recently gave birth to flow virometry, a new way to detect, analyze, and characterize single viral particles. Detection of viruses by flow cytometry is possible due to improvements in current flow cytometers, calibration, and tuning methods. We summarize the recent birth and novel uses of flow virometry and the progressive evolution of this tool to advance the field of virology. We also discuss the various flow virometry methods used to identify and analyze viruses. We briefly summarize other applications of flow virometry, including: virus detection, quantification, population discrimination, and viral particles' antigenic properties. Finally, we summarize how viral sorting will allow further progress of flow virometry to relate viral surface characteristics to infectivity properties.
摘要
在过去的几十年中，流式细胞术重新定义了生物学领域，极大地增强了我们对细胞、免疫学和微生物学的理解。流式细胞术最近催生了流式病毒计量学，这是一种检测、分析和表征单个病毒颗粒的新方法。由于当前流式细胞仪、校准和调整方法的改进，才使得通过流式细胞术检测病毒成为可能。我们总结了流式病毒计量学的最新发展和新用途，以及该工具的逐步发展如何推动病毒学领域的发展。我们还讨论了用于识别和分析病毒的各种流式病毒计量学方法。我们简要总结了流式病毒计量学的其他应用，包括病毒检测、定量、群体区分和病毒颗粒的抗原特性。最后，我们总结了病毒分选如何使流式病毒计量学进一步发展，将病毒表面特性与感染性特性联系起来。
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