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卵巢卵泡的转录组分析揭示了与蛋鸡产蛋率增减相关的潜在关键基因。

Transcriptome Analysis of Ovarian Follicles Reveals Potential Pivotal Genes Associated With Increased and Decreased Rates of Chicken Egg Production.

作者信息

Chen Xiaoxia, Sun Xue, Chimbaka Ignatius Musenge, Qin Ning, Xu Xiaoxing, Liswaniso Simushi, Xu Rifu, Gonzalez John Michael

机构信息

Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.

Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun, China.

出版信息

Front Genet. 2021 Mar 10;12:622751. doi: 10.3389/fgene.2021.622751. eCollection 2021.

DOI:10.3389/fgene.2021.622751
PMID:33777097
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7987945/
Abstract

Egg production is an important economic trait in the commercial poultry industry. Ovarian follicle development plays a pivotal role in regulation of laying hen performance and reproductive physiology. However, the key genes and signaling pathways involved in the various-stages of laying hen follicular development remain poorly understood. In this study, transcriptomes of ovarian follicles at three developmental stages, the large white follicle (LWF), small yellow follicle (SYF), and large yellow follicle (LYF), were comparatively analyzed in hens with high (HR) and low (LR) egg-laying rates by RNA-sequencing. Eighteen cDNA libraries were constructed and a total of 236, 544, and 386 unigenes were significantly differentially expressed in the LWF, SYF, and LYF follicles of HR and LR hens, respectively. Among them, 47 co-transcribed differentially expressed genes (DEGs) in LWF and SYF, 68 co-expressed DEGs in SYF and LYF, and 54 co-expressed DEGs in LWF and LYF were mined. Thirteen co-expressed DEGs were found in LWF, SYF, and LYF follicles. Eighteen candidate genes, including , , , , , , , , , , , , , , , , , and were identified to be potentially related to egg production. Furthermore, Kyoto Encyclopedia of Genes and Genomes analysis indicated neuroactive ligand-receptor interaction, cell adhesion molecules, peroxisome proliferator-activated receptor pathway, and cAMP signaling pathway might elicit an important role in formation of egg-laying traits by influencing ovarian follicle development. This study represents the first transcriptome analysis of various-sized follicles between HR and LR hens. These results provide useful molecular evidence for elucidating the genetic mechanism underlying ovarian follicle development associated with egg production in chicken.

摘要

产蛋是商业家禽业的一项重要经济性状。卵巢卵泡发育在蛋鸡生产性能和生殖生理调节中起着关键作用。然而，参与蛋鸡卵泡发育各个阶段的关键基因和信号通路仍知之甚少。在本研究中，通过RNA测序对高（HR）、低（LR）产蛋率母鸡的三个发育阶段（大白卵泡（LWF）、小黄卵泡（SYF）和大黄卵泡（LYF））的卵巢卵泡转录组进行了比较分析。构建了18个cDNA文库，HR和LR母鸡的LWF、SYF和LYF卵泡中分别有236、544和386个单基因显著差异表达。其中，挖掘出LWF和SYF中47个共转录差异表达基因（DEGs）、SYF和LYF中68个共表达DEGs以及LWF和LYF中54个共表达DEGs。在LWF、SYF和LYF卵泡中发现了13个共表达DEGs。鉴定出18个候选基因，包括……，它们可能与产蛋有关。此外，京都基因与基因组百科全书分析表明，神经活性配体-受体相互作用、细胞粘附分子、过氧化物酶体增殖物激活受体途径和cAMP信号通路可能通过影响卵巢卵泡发育在产蛋性状形成中发挥重要作用。本研究是首次对HR和LR母鸡不同大小卵泡进行转录组分析。这些结果为阐明鸡卵巢卵泡发育与产蛋相关的遗传机制提供了有用的分子证据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78a1/7987945/8b580e332891/fgene-12-622751-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78a1/7987945/0533d909db24/fgene-12-622751-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78a1/7987945/71d6cec6e490/fgene-12-622751-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78a1/7987945/536179ab7bec/fgene-12-622751-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78a1/7987945/2e1f868b5e82/fgene-12-622751-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78a1/7987945/7725c2ee9f0b/fgene-12-622751-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78a1/7987945/7ccbf69eb3fa/fgene-12-622751-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78a1/7987945/8b580e332891/fgene-12-622751-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78a1/7987945/0533d909db24/fgene-12-622751-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78a1/7987945/71d6cec6e490/fgene-12-622751-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78a1/7987945/536179ab7bec/fgene-12-622751-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78a1/7987945/2e1f868b5e82/fgene-12-622751-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78a1/7987945/7725c2ee9f0b/fgene-12-622751-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78a1/7987945/7ccbf69eb3fa/fgene-12-622751-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78a1/7987945/8b580e332891/fgene-12-622751-g007.jpg

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