Nursyirwani Nursyirwani, Asmara Widya, Wahyuni Agnesia Endang Tri Hastuti, Triyanto Triyanto, Fauzi Muhammad, Muchlisin Zainal Abidin
Faculty of Fishery and Marine Science, Universitas Riau, Pekanbaru, Indonesia.
Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia.
F1000Res. 2017 Nov 10;6:1984. doi: 10.12688/f1000research.12734.1. eCollection 2017.
Lactic acid bacteria (LAB) have been isolated successfully from the tiger grouper intestine. However, their genus or species have not been identified. Therefore, the objective of the present study was to characterize the three isolated LAB (KSBU-12C, KSBU-5Da, and KSBU-9) based on their phenotype and genotype. The LAB phenotype was examined by observing morphological features including cell morphology, spore production and motility. The physiological tests were performed in 6.5% NaCl at the temperatures of 10 C and 45 C, and the biochemical tests were evaluated based on the production of enzymes catalase, oxidase and arginine dehydrolase, following the Standard Analytical Profile Index, API 50 CH kit. The genotype was examined based on 16S rDNA gene sequence analysis , and the products were analyzed using the BLAST (Basic Local Alignment Search Tool) NCBI database. The three isolates (KSBU-5Da, KSBU-12C, and KSBU-9) were categorized into the genus . 16S rDNA sequence analysis indicated that the isolates had 99% similarity to ATCC 9790, registered in GenBank with accession number NR_075022.1. It was concluded that the three LAB isolates taken from the tiger grouper are .
乳酸菌（LAB）已成功从老虎斑肠道中分离出来。然而，它们的属或种尚未确定。因此，本研究的目的是基于其表型和基因型对三种分离出的乳酸菌（KSBU - 12C、KSBU - 5Da和KSBU - 9）进行特征描述。通过观察包括细胞形态、孢子产生和运动性在内的形态学特征来检测乳酸菌的表型。生理测试在6.5%氯化钠中于10℃和45℃的温度下进行，生化测试根据过氧化氢酶、氧化酶和精氨酸脱氨酶的产生情况进行评估，遵循标准分析谱指数，即API 50 CH试剂盒。基于16S rDNA基因序列分析来检测基因型，并使用BLAST（基本局部比对搜索工具）NCBI数据库对产物进行分析。这三种分离株（KSBU - 5Da、KSBU - 12C和KSBU - 9）被归类到属。16S rDNA序列分析表明，这些分离株与保藏号为NR_075022.1登记在GenBank中的ATCC 9790有99%的相似性。得出的结论是，从老虎斑中分离出的这三种乳酸菌分离株是 。
