Meny Paulina, Menéndez Clara, Quintero Jair, Hernández Elba, Ríos Cristina, Balassiano Ilana Teruszkin, Trindade Camilla Nunes Dos Reis, Vital-Brazil Juliana Magalhães, Ramos Tatiane Mendes Varela, Ashfield Natalia, Feble Camila, Avila Esthefani, Schelotto Felipe, Varela Gustavo
Universidad de la República, Uruguay.
Instituto Osvaldo Cruz, Centro de Referência Nacional para Leptospirose, WHO Collaborating Center for Leptospirosis, Laboratório de Zoonoses Bacterianas, Rio de Janeiro, Rio de Janeiro, Brazil.
Rev Inst Med Trop Sao Paulo. 2017 Dec 21;59:e79. doi: 10.1590/S1678-9946201759079.
Laboratory diagnosis of human leptospirosis usually relies on indirect methods exploring specific immune response. Isolation and identification of the involved strains are cumbersome, but can provide biological resources for pathogenic studies and relevant information for guiding prevention and control measures. The aim of the research we are hereby reporting was the characterization of Leptospira isolates obtained from humans and the environment in Uruguay. Blood cultures were performed from early samples of 302 Uruguayan patients, mainly rural workers, and from 36 water samples taken from their living or working environments. Eight human isolates and seven environmental isolates were obtained and analyzed by end point Polymerase Chain Reaction (PCR), Multilocus Variable Number of Tandem Repeat Analysis (MLVA) and other molecular methods. Human isolates corresponded to several serogroups and serovars of Leptospira interrogans and Leptospira kirschneri species, probably reflecting the infection with similar involved Leptospira species and serovars of an extended animal reservoir in rural settings of the country, mostly dedicated to meat and dairy production. Culture-positive patients were older than usually affected workers, and presented signs and symptoms of severe illness. A high organic and circulating bacterial burden may explain an easier positive result from these workers' samples. Environmental isolates were mainly identified as Leptospira biflexa strains, with a single L. meyeri isolate of uncertain significance.
人类钩端螺旋体病的实验室诊断通常依赖于探索特异性免疫反应的间接方法。所涉菌株的分离和鉴定过程繁琐，但可为致病性研究提供生物资源，并为指导预防和控制措施提供相关信息。我们在此报告的这项研究的目的是对从乌拉圭人和环境中分离出的钩端螺旋体进行特征分析。对302名乌拉圭患者（主要是农民工）的早期样本以及从他们的生活或工作环境中采集的36份水样进行了血培养。获得了8株人源分离株和7株环境分离株，并通过终点聚合酶链反应（PCR）、多位点可变数目串联重复序列分析（MLVA）及其他分子方法进行了分析。人源分离株对应于问号钩端螺旋体和克氏钩端螺旋体的多个血清群和血清型，这可能反映出该国农村地区广泛的动物宿主中类似的钩端螺旋体种类和血清型感染情况，这些地区主要从事肉类和奶制品生产。培养阳性的患者比通常受影响的工人年龄更大，且表现出严重疾病的体征和症状。高有机负荷和循环细菌载量可能解释了这些工人样本更容易获得阳性结果的原因。环境分离株主要鉴定为双曲钩端螺旋体菌株，有1株意义不明的迈氏钩端螺旋体分离株。
