Laudone V P, Morgan T R, Huryk R F, Heston W D, Fair W R
Department of Surgery, Urology Service, Memorial Sloan-Kettering Cancer Center, New York, New York.
J Urol. 1989 Apr;141(4):965-8. doi: 10.1016/s0022-5347(17)41079-2.
In vivo and in vitro experimentation with high energy shock waves (HESW) is necessary to further our understanding of the biologic effects and potential application of this novel energy form. Factors are identified which are critical to the design and subsequent interpretation of HESW experimentation. First, the nature of the containment vessel and the presence or absence of acoustic interfaces are shown to significantly alter the outcome of cell suspension experiments. Second, the effects of HESW are shown to differ markedly for cells in suspension versus cells in tissue making comparisons between the two uncertain. Finally, the need for appropriate negative controls is demonstrated with in vivo experiments to control for the generalized toxicity which occurs when small animals are exposed to such an intense force distributed over a relatively large area. These findings affect the interpretation of previously reported work which investigated the cytotoxic potential of HESW.
为了进一步了解这种新型能量形式的生物学效应和潜在应用，有必要对高能冲击波（HESW）进行体内和体外实验。确定了对HESW实验设计及后续解释至关重要的因素。首先，已表明容纳容器的性质以及声学界面的存在与否会显著改变细胞悬浮液实验的结果。其次，对于悬浮细胞和组织中的细胞，HESW的效应存在显著差异，这使得两者之间的比较变得不确定。最后，体内实验证明了需要适当的阴性对照，以控制小动物暴露于分布在相对大面积上的如此强烈力量时发生的全身毒性。这些发现影响了先前报道的关于研究HESW细胞毒性潜力的工作的解释。
