Imada T, Kawamura H, Nishimori T, Murata H, Narita M, Honda Y, Ishigaki K
Nihon Juigaku Zasshi. 1989 Feb;51(1):96-104. doi: 10.1292/jvms1939.51.96.
The pathogenicity and immunogenicity of vaccinia virus were examined in order to evaluate the possibility of its application as a recombinant viral vaccine in pigs. Following scarification inoculation with vaccinia virus, a mild reddish papulation developed only on the scarified part of the skin. No symptoms of illness such as fever or stunting were noted. Vaccinia virus was recovered in titers from scarified skin 4 and 7 days after inoculation. Control piglets cohabited with inoculated animals remained normal for the whole 5 week observation period. Hemagglutination inhibition and indirect immunofluorescence tests detected antibodies against vaccinia virus in the inoculated piglets, whereas no anti-vaccinia virus antibodies were detected in the contact control animals. Antigen-induced blastogenic tests of peripheral blood lymphocytes from animals, revealed that lymphocytes obtained from inoculated donors 5 weeks after inoculation, had a higher stimulation index (P less than 0.05) than did those from uninoculated piglets. These results suggested that vaccinia virus would be useful as a recombinant viral vector for pigs.
为了评估痘苗病毒作为猪用重组病毒疫苗的应用可能性，对其致病性和免疫原性进行了检测。用痘苗病毒划痕接种后，仅在皮肤划痕部位出现轻度微红丘疹。未观察到发热或发育迟缓等疾病症状。接种后4天和7天，从划痕皮肤中回收了痘苗病毒。在整个5周的观察期内，与接种动物同居的对照仔猪保持正常。血凝抑制试验和间接免疫荧光试验在接种仔猪中检测到抗痘苗病毒抗体，而在接触对照动物中未检测到抗痘苗病毒抗体。对接种动物外周血淋巴细胞进行抗原诱导的增殖试验，结果显示，接种5周后从接种供体获得的淋巴细胞的刺激指数高于未接种仔猪（P<0.05）。这些结果表明，痘苗病毒可作为猪用重组病毒载体。
