Department of Chemistry, University of Bergen, Allégt. 41, N-5007 Bergen, Norway.
Department of Geography, University of Bergen, Fosswinckelsft. 6, N-5020 Bergen, Norway.
Molecules. 2017 Dec 22;23(1):16. doi: 10.3390/molecules23010016.
Herein, the polyphenolic content in extracts of (Petagna) Grande and L.was fully characterized for the first time. High amounts of the main compound chicoric acid () (≤30.2 ± 4.3 mg/g) were found in both species. In addition, eight flavonoids, namely the 3--glucopyranosides and 3--galactopyranosides, as well as malonylated 3--glycosides of quercetin and isorhamnetin, were isolated and identified. The antioxidant activity of extracts and isolated compounds was investigated spectrophotometrically by a 1,1-diphenyl-2-picrylhydrazyl (DPPH·) radical scavenging assay. IC values were 31.8-175.7 μg/mL for extracts and 12.1-88.4 μg/mL for isolated flavonoids. Both individual and total phenolic and flavonoid content were quantified in crude extracts using analytical HPLC. The relative high amount of total flavonoids ranged from 5.9 to 14.7 mg/g in both species, with concentrations of individual flavonoids ranging from 0.4 to 2.9 mg/g dry weight. The content of chicoric acid was twofold more in than in . Seasonal variation of the quantitative content in was examined. Total flavonoid content ranged from 8.4 mg/g in October to 14.7 mg/g in August, whereas the highest concentration of chicoric acid was observed in March (29.2 mg/g).
本文首次对（Petagna） Grande 和 L. 的提取物中的多酚含量进行了全面表征。在这两个物种中，均发现了大量的主要化合物 chicoric 酸（）（≤30.2 ± 4.3 mg/g）。此外，还分离并鉴定了八种类黄酮，即 3--葡萄糖苷和 3--半乳糖苷，以及槲皮素和异鼠李素的丙二酰基 3--糖苷。通过 1,1-二苯基-2-苦基肼（DPPH·）自由基清除测定法，分光光度法研究了提取物和分离化合物的抗氧化活性。提取物的 IC 值为 31.8-175.7 μg/mL，分离的类黄酮的 IC 值为 12.1-88.4 μg/mL。使用分析型 HPLC 对粗提取物中的总酚和类黄酮的含量进行了定量分析。两种物种的总类黄酮含量相对较高，范围为 5.9-14.7 mg/g，各单体类黄酮的浓度范围为 0.4-2.9 mg/g 干重。与 L. 相比， 中的 chicoric 酸含量高两倍。还检查了 的定量含量的季节性变化。总类黄酮含量范围为 10 月的 8.4 mg/g 到 8 月的 14.7 mg/g，而 chicoric 酸的最高浓度出现在 3 月（29.2 mg/g）。
