College of Biosystems Engineering and Food Science , Zhejiang University , Hangzhou 310058 , China.
College of Animal Sciences , Zhejiang University , Hangzhou 310058 , China.
Anal Chem. 2020 Jan 21;92(2):1818-1825. doi: 10.1021/acs.analchem.9b03644. Epub 2019 Dec 3.
Nanochannels hold great prospects in intelligent systems; however, current research focuses on the inner space of the nanochannel while the outer surface is rarely explored. Here, we report on a cooperation mode of the outer surface and inner space of the nanochannel using an integrated nanochannel-electrode (INCE) and its application as a separation-detection system for rapid and facile detection of foodborne bacteria. Unlike conventional nanochannel systems, the INCE integrates two electrodes as a sensitive electrochemical interface and the nanochannel itself as nanofilter, generating a novel separation-detection system. The system is examined in a biosensing strategy based on magnetic nanoparticles (MNPs). () is taken as the target due to its severe threat to human health and food safety. By electrochemically probing the MNPs- complex themselves on the surface of INCE, this method eliminates the requirement on additional signal labels. The biosensor presents a linear detection range from 10 to 10 CFU mL and a limit of detection of 50 CFU mL, being comparable or even better than those of analogues with complicated signal amplification designs. Moreover, the biosensor exhibits good specificity against four types of interfering bacteria. This concept may bring new insight into the development of nanochannel research and contribute a new way to the fields of separation and detection.
纳米通道在智能系统中有很大的应用前景；然而，目前的研究主要集中在纳米通道的内部空间，而很少探索其外部表面。在这里，我们报告了一种使用集成纳米通道-电极（INCE）的纳米通道内外表面协同作用的模式，以及将其作为一种快速简便的食源性病原体检测分离-检测系统的应用。与传统的纳米通道系统不同，INCE 将两个电极集成作为一个敏感的电化学界面，而纳米通道本身作为纳米滤波器，产生了一种新型的分离-检测系统。该系统在基于磁性纳米粒子（MNPs）的生物传感策略中进行了检验。由于其对人类健康和食品安全的严重威胁，我们选择作为目标。通过电化学探测 INCE 表面上的 MNPs-复合物本身，该方法消除了对额外信号标记的需求。该生物传感器的线性检测范围为 10 至 10 CFU mL，检测限为 50 CFU mL，与具有复杂信号放大设计的类似物相当，甚至更好。此外，该生物传感器对四种类型的干扰细菌表现出良好的特异性。这一概念可能为纳米通道研究的发展带来新的见解，并为分离和检测领域提供一种新的方法。
