Ye Peng, Yu Bin, Deng Jiang, She Rong-Feng, Huang Wen-Liang
Department of Orthopaedics, Nanfang Hospital, First School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
Department of Orthopaedic Trauma, The Southern Hospital Affiliated to Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
Exp Ther Med. 2017 Dec;14(6):5547-5553. doi: 10.3892/etm.2017.5231. Epub 2017 Sep 29.
Silk fibroin (SF), chitosan (CS) and nano-hydroxyapatite (nHA) possess excellent biocompatibility, thus, these were used to construct a SF/CS/nHA composite scaffold. Previously published results identified that this material exhibited satisfactory physical and chemical properties, and therefore qualified as a repair material in bone tissue engineering. The aim of the present study was to investigate the capacity and mechanism of this composite scaffold in repairing bone defects. In total, 45 New Zealand white rabbits were used to model defect in the right radial bone. A radial bone defect was induced, and rabbits were divided into the following treatment groups (n=15 in each): Group A, in which the SF/CS/nHA scaffold was implanted; group B, in which the SF/CS scaffold was implanted; and group C, in which rabbits did not receive subsequent treatment. X-ray scanning, specimen observation and histopathological examination were implemented at 1, 2, 3 and 4 months after modeling, in order to evaluate the osteogenic capacity and mechanism. At 1 month after modeling, the bone density shadow in the X-ray scan was darker in group A as compared with that in group B. Observation of the pathological specimens indicated that normal bone tissues partially replaced the scaffold. At 2 months, the bone density shadow of group A was similar to normal bone tissues, and normal tissue began to replace the scaffold. At 3-4 months after modeling, the X-ray scan and histopathological observation indicated that the normal bone tissues completely replaced the scaffold in group A, with an unobstructed marrow cavity. However, the bone mass of group B was lower in comparison with that of group A. The bone defect induced in group C was filled with fibrous connective tissues. Therefore, it was concluded that the SF/CS/nHA composite scaffold may be a promising material for bone tissue engineering.
丝素蛋白（SF）、壳聚糖（CS）和纳米羟基磷灰石（nHA）具有优异的生物相容性，因此，这些材料被用于构建SF/CS/nHA复合支架。先前发表的结果表明，这种材料具有令人满意的物理和化学性质，因此有资格作为骨组织工程中的修复材料。本研究的目的是探讨这种复合支架修复骨缺损的能力和机制。总共使用45只新西兰白兔建立右侧桡骨缺损模型。诱导桡骨缺损后，将兔子分为以下治疗组（每组n = 15）：A组，植入SF/CS/nHA支架；B组，植入SF/CS支架；C组，兔子不接受后续治疗。在建模后1、2、3和4个月进行X射线扫描、标本观察和组织病理学检查，以评估成骨能力和机制。建模后1个月，A组X射线扫描中的骨密度阴影比B组更暗。病理标本观察表明，正常骨组织部分替代了支架。2个月时，A组的骨密度阴影与正常骨组织相似，正常组织开始替代支架。建模后3 - 4个月，X射线扫描和组织病理学观察表明，A组正常骨组织完全替代了支架，骨髓腔通畅。然而，B组的骨量低于A组。C组诱导的骨缺损被纤维结缔组织填充。因此，得出结论，SF/CS/nHA复合支架可能是骨组织工程中有前景的材料。
