Jang Bian, Xu Li, Moorthy Madhappan S, Zhang Wei, Zeng Ling, Kang Mingyeong, Kwak Minseok, Oh Junghwan, Jin Jun-O
Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University, Shanghai, China.
Marine-Integrated Bionics Research Center, Pukyong National University, Busan, South Korea.
Oncotarget. 2017 Nov 6;8(62):105584-105595. doi: 10.18632/oncotarget.22331. eCollection 2017 Dec 1.
To meet the ultimate goal of cancer therapy, which is treating not only the primary tumor but also preventing metastatic cancer, the concept of combining immunotherapy with photothermal therapy (PTT) is gaining great interest. Here, we studied the new material, lipopolysaccharide (LPS) coated copper sulfide nanoparticles (LPS-CuS), for the immuno-photothermal therapy. We evaluated the effect of LPS-CuS for induction of apoptosis of CT26 cells and activation of dendritic cells. Moreover, the LPS-CuS and laser irradiation was examined anti-metastasis effect by liver metastasis model mouse . Through PTT, LPS-CuS induced elimination of CT26 tumor in BALB/c mice, which produced cancer antigens. In addition, released LPS and cancer antigen by PTT promoted dendritic cell activation in tumor draining lymph node (drLN), and consequently, enhanced the tumor antigen-specific immune responses. Finally, the primary tumor cured mice by LPS-CuS-mediated PTT completely resisted secondary tumor injection in the spleen and also prevented liver metastasis. Our results demonstrated the potential usage of LPS-CuS for the immuno-photothermal therapy against various types of cancer by showing the clear elimination of primary colon carcinoma with complete prevention of spleen and liver metastasis.
为了实现癌症治疗的最终目标，即不仅治疗原发性肿瘤，还要预防转移性癌症，免疫疗法与光热疗法（PTT）相结合的概念正引起人们极大的兴趣。在此，我们研究了用于免疫光热疗法的新材料——脂多糖（LPS）包覆的硫化铜纳米颗粒（LPS-CuS）。我们评估了LPS-CuS诱导CT26细胞凋亡和激活树突状细胞的效果。此外，通过肝转移模型小鼠研究了LPS-CuS与激光照射的抗转移作用。通过光热疗法，LPS-CuS诱导BALB/c小鼠体内产生癌症抗原的CT26肿瘤消退。此外，光热疗法释放的LPS和癌症抗原促进了肿瘤引流淋巴结（drLN）中树突状细胞的活化，从而增强了肿瘤抗原特异性免疫反应。最后，通过LPS-CuS介导的光热疗法治愈原发性肿瘤的小鼠完全抵抗了脾脏中的继发性肿瘤注射，并且还预防了肝转移。我们的结果通过显示原发性结肠癌的明显消退以及脾脏和肝脏转移的完全预防，证明了LPS-CuS在针对各种类型癌症的免疫光热疗法中的潜在用途。
