Chemical Constituents of the Ethyl Acetate Extract from Diaphragma juglandis Fructus and Their Inhibitory Activity on Nitric Oxide Production In Vitro.

fructus contains various bioactive constituents. Fourteen compounds were isolated from fructus by preparative high performance liquid chromatography (pre-HPLC) and high-speed counter-current chromatography (HSCCC). Their structures were identified by nuclear magnetic resonance (NMR) and electrospray ionization mass spectrometry (ESI-MS). Compounds (+)-dehydrovomifoliol (), (6,9)-9-hydroxymegastigman-4-en-3-one () and (6,9)-9-hydroxymegastigman-4-en-3-one () are found from L. for the first time. Compounds dihydrophaseic acid (), blumenol B () and (4)-4-hydroxy-1-tetralone () are isolated from fructus for the first time. The anti-inflammatory effects of isolated compounds were evaluated by an in vitro model of lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Compounds gallic acid (), ethyl gallate () and (+)-dehydrovomifoliol () exhibited inhibitory activity on the nitric oxide production of RAW 264.7 at a concentration of 25 μM. The result indicated that the combination HSCCC with pre-HPLC is an effective way for compound separation and purification. And fructus constituents have the potential for the treatment of inflammatory-related diseases.