Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), 19 Keyuan Street, Jinan 250014, China.
College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Street, Taian 271018, China.
Molecules. 2017 Dec 29;23(1):72. doi: 10.3390/molecules23010072.
fructus contains various bioactive constituents. Fourteen compounds were isolated from fructus by preparative high performance liquid chromatography (pre-HPLC) and high-speed counter-current chromatography (HSCCC). Their structures were identified by nuclear magnetic resonance (NMR) and electrospray ionization mass spectrometry (ESI-MS). Compounds (+)-dehydrovomifoliol (), (6,9)-9-hydroxymegastigman-4-en-3-one () and (6,9)-9-hydroxymegastigman-4-en-3-one () are found from L. for the first time. Compounds dihydrophaseic acid (), blumenol B () and (4)-4-hydroxy-1-tetralone () are isolated from fructus for the first time. The anti-inflammatory effects of isolated compounds were evaluated by an in vitro model of lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Compounds gallic acid (), ethyl gallate () and (+)-dehydrovomifoliol () exhibited inhibitory activity on the nitric oxide production of RAW 264.7 at a concentration of 25 μM. The result indicated that the combination HSCCC with pre-HPLC is an effective way for compound separation and purification. And fructus constituents have the potential for the treatment of inflammatory-related diseases.
五味子果实含有多种生物活性成分。通过制备高效液相色谱（pre-HPLC）和高速逆流色谱（HSCCC）从五味子中分离出 14 种化合物。通过核磁共振（NMR）和电喷雾电离质谱（ESI-MS）鉴定了它们的结构。（+）-去氢五味子醇（）、（6,9）-9-羟基麦角甾-4-烯-3-酮（）和（6,9）-9-羟基麦角甾-4-烯-3-酮（）是首次从五味子中分离得到的化合物。二氢没食子酸（）、紫丁香苷 B（）和（4）-4-羟基-1-四氢酮（）是首次从五味子果实中分离得到的化合物。采用脂多糖（LPS）刺激 RAW 264.7 巨噬细胞的体外模型评价了分离化合物的抗炎作用。化合物没食子酸（）、没食子酸乙酯（）和（+）-去氢五味子醇（）在 25 μM 浓度下对 RAW 264.7 的一氧化氮生成具有抑制活性。结果表明，HSCCC 与 pre-HPLC 相结合是化合物分离和纯化的有效方法。五味子果实成分具有治疗炎症相关疾病的潜力。
