Melish M E, Murata S, Fukunaga C, Frogner K, McKissick C
Department of Pediatrics, John A. Burns School of Medicine, University of Hawaii, Kapiolani Medical Center, Honolulu 96826.
Rev Infect Dis. 1989 Jan-Feb;11 Suppl 1:S238-46; discussion S246-7. doi: 10.1093/clinids/11.supplement_1.s238.
The effects of tampon composition, inoculum size, and simulated menses on production of toxic shock syndrome toxin 1 (TSST-1) and toxic shock syndrome (TSS) were evaluated in a rabbit model that simulates tampon use in humans. Three small generic compressed-fiber tampons were successively inserted vaginally (remained in place 4.5 hours x 2; overnight x 1). Tampon no. 1 was inoculated with live TSST-1-positive staphylococci plus 5 mL of saline or simulated menses (defibrinated rabbit blood plus 2.5 g of bovine serum albumin/dL) immediately after insertion; saline or simulated menses alone were used with tampons no. 2 and 3. The vagina was washed after removal of tampon no. 3. TSS-like illness was produced consistently in animals with carboxymethyl cellulose/polyester foam tampons, which supported higher organism counts and greater TSST-1 production in association with subsequent tampons. Cotton and rayon tampons were not associated with as much clinical illness, organism growth, or TSST-1 production. Simulated menses supported toxin production and clinical illness when the inoculum was one-tenth that required for controls. Sham tampon insertion was associated with TSS-like illness in two of 10 rabbits; thus, other factors may promote TSS in the absence of vaginal tampons. This model reliability reproduces menstrual TSS, since one-time vaginal inoculation with TSST-1-positive staphylococci in the presence of blood and certain tampons leads to TSS, and may be useful in evaluating catamenial products and in understanding other factors important in TSST-1 production in vivo and the development of TSS.
在一个模拟人类使用卫生棉条的兔模型中，评估了卫生棉条成分、接种量和模拟月经对毒性休克综合征毒素1（TSST - 1）产生及毒性休克综合征（TSS）的影响。将三个小型普通压缩纤维卫生棉条依次经阴道插入（放置4.5小时×2次；过夜×1次）。1号卫生棉条在插入后立即接种活的TSST - 1阳性葡萄球菌加5毫升生理盐水或模拟月经（去纤维蛋白兔血加2.5克牛血清白蛋白/分升）；2号和3号卫生棉条单独使用生理盐水或模拟月经。在取出3号卫生棉条后冲洗阴道。使用羧甲基纤维素/聚酯泡沫卫生棉条的动物 consistently出现类TSS疾病，这与随后的卫生棉条导致更高的菌数和更多的TSST - 1产生有关。棉和人造纤维卫生棉条与较少的临床疾病、细菌生长或TSST - 1产生无关。当接种量为对照组所需接种量的十分之一时，模拟月经支持毒素产生和临床疾病。假插入卫生棉条在10只兔子中有2只出现类TSS疾病；因此，在没有阴道卫生棉条的情况下，其他因素可能会促进TSS的发生。该模型可靠性高，可重现月经性TSS，因为在有血液和某些卫生棉条存在的情况下，一次性经阴道接种TSST - 1阳性葡萄球菌会导致TSS，该模型可能有助于评估经期用品，并有助于了解体内TSST - 1产生及TSS发展过程中其他重要因素。 （注：原文中“consistently”这个词翻译时感觉原译文此处可能有误，但按照要求未做修改，推测可能是“持续地”之类的意思，结合语境不太好准确理解其在原文中的准确含义）
