Laboratoire de recherche et de protection des plantes, URPP- INRA-Meknès, Meknes, Morocco.
Laboratoire de Botanique, Biotechnologie, et Protection des Plantes, Faculté des Sciences, Kenitra, Morocco.
Environ Sci Pollut Res Int. 2018 Oct;25(30):29943-29952. doi: 10.1007/s11356-017-1008-9. Epub 2017 Dec 29.
Allorhizobium (Agrobacterium) vitis is a host-specific pathogenic bacterium that causes grapevine crown gall disease, affecting vine growth and production worldwide. The antibacterial activities of different aromatic plant essential oils were tested in vitro and in planta against A. vitis. Among the essential oils tested, those of Origanum compactum and Thymus vulgaris showed the most significant in vitro antibacterial activities, with a MIC of 0.156 and 0.312 mg/mL, respectively. A synergistic effect of these two essential oils (1:1) was observed and confirmed by the checkerboard test. Carvacrol (61.8%) and thymol (47.8%) are, respectively, the major compounds in the essential oils of O. compactum and T. vulgaris and they have been shown to be largely responsible for the antibacterial activities of their corresponding essential oils. Results obtained in vitro were reinforced by an in planta pathogenicity test. A mixture of O. compactum and T. vulgaris essential oils (1:1), inoculated into the injured stem of a tomato plant and a grapevine at 0.312 mg/mL as a preventive treatment, reduced both the number of plants developing gall symptoms and the size of the tumors.
根瘤农杆菌（根癌农杆菌）是一种宿主特异性的致病细菌，可引起葡萄冠瘿病，影响全球葡萄的生长和产量。本研究测试了不同芳香植物精油对 A. vitis 的体外和体内抗菌活性。在测试的精油中，牛至（Oreganum compactum）和普通百里香（Thymus vulgaris）精油表现出最强的体外抗菌活性，MIC 分别为 0.156 和 0.312mg/mL。棋盘试验证实了这两种精油（1:1）具有协同作用。香芹酚（61.8%）和百里酚（47.8%）分别是牛至和普通百里香精油的主要成分，它们被证明对相应精油的抗菌活性起主要作用。体外试验结果通过体内致病性试验得到了加强。将牛至和普通百里香精油（1:1）混合物以 0.312mg/mL 的浓度作为预防处理，接种到番茄植株和葡萄受伤的茎中，可减少出现瘿瘤症状的植物数量和肿瘤大小。
