Engevik Kristen A, Matthis Andrea L, Montrose Marshall H, Aihara Eitaro
Department of Pharmacology and Systems Physiology, University of Cincinnati, Cincinnati, OH, USA.
Methods Mol Biol. 2018;1734:71-81. doi: 10.1007/978-1-4939-7604-1_8.
The advent of the gastric organoid culture system has provided a new model to emulate native epithelial tissue in vitro. Gastric organoids grow from isolated epithelial stem cells and develop into three dimensional structures that can be used to study host physiology. Here we describe current laboratory protocols for growing gastric organoids and the microinjection of pathogens such as Helicobacter pylori into the lumen of gastric organoids in order to study the cellular response following infection.
胃类器官培养系统的出现为在体外模拟天然上皮组织提供了一种新模型。胃类器官由分离的上皮干细胞生长而来，并发育成三维结构，可用于研究宿主生理学。在这里，我们描述了目前培养胃类器官以及将幽门螺杆菌等病原体显微注射到胃类器官管腔中以研究感染后细胞反应的实验室方案。
