嫩度不同基因型内洛尔牛牛肉的蛋白质组变化。
Proteome changes of beef in Nellore cattle with different genotypes for tenderness.
机构信息
Department of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, 225, Duque de Caxias, 13635-900 Pirasssununga, SP, Brazil.
Department of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, 225, Duque de Caxias, 13635-900 Pirasssununga, SP, Brazil.
出版信息
Meat Sci. 2018 Apr;138:1-9. doi: 10.1016/j.meatsci.2017.12.006. Epub 2017 Dec 13.
In the present study, 155 Nellore cattle were genotyped for the CAPN4751 and UOGCAST polymorphisms and phenotyped for shear force (SF) at 1, 7 and 14days aging. The effects of different genotypic combinations were evaluated on the Longissimus muscle proteomic profile using 2DE and mass spectrometry. A significant association was found between genotypes for UOGCAST and CAPN4751 and meat tenderness. The CC genotype for both markers was favorable for lesser shear force than TT. A total of 40 spots showed significant differential expression profiles (P<0.05), of which eight had a main effect for the CAPN4751 marker, 11 for UOGCAST, two for both markers, and 19 had interactions between markers, including myosin (MYL1, MYL2, MYLPF and MYL6B), actin (ACTA1 and CAPZβ), troponin (TNNT1 and TNNT3) and heat shock proteins (HSPB6, HSPB1 and HSP70-2). The results demonstrated that UOGCAST and CAPN4751 genotypes led to variability on the expression of proteins that are involved in muscle metabolism, and consequently affect meat tenderness.
在本研究中，对 155 头尼洛拉牛进行 CAPN4751 和 UOGCAST 多态性基因分型，并在 1、7 和 14 天老化时进行剪切力 (SF) 表型分析。使用 2DE 和质谱法评估不同基因型组合对最长肌蛋白质组图谱的影响。UOGCAST 和 CAPN4751 基因座的基因型与肉质嫩度之间存在显著关联。两个标记的 CC 基因型比 TT 基因型的剪切力更小。共发现 40 个斑点表现出显著的差异表达谱（P<0.05），其中 8 个与 CAPN4751 标记有主要效应，11 个与 UOGCAST 标记有主要效应，2 个与两个标记都有主要效应，19 个与标记之间的相互作用有关，包括肌球蛋白 (MYL1、MYL2、MYLPF 和 MYL6B)、肌动蛋白 (ACTA1 和 CAPZβ)、肌钙蛋白 (TNNT1 和 TNNT3) 和热休克蛋白 (HSPB6、HSPB1 和 HSP70-2)。结果表明，UOGCAST 和 CAPN4751 基因型导致参与肌肉代谢的蛋白质表达发生变化，从而影响肉质嫩度。
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