Glycomyces tritici sp. nov., isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Glycomyces.

A novel actinomycete strain, designated NEAU-C2, was isolated from rhizosphere soil of wheat (Triticum aestivum L.), and subjected to a polyphasic taxonomic study. Morphological and chemotaxonomic properties of this strain were consistent with the description of the genus Glycomyces. Growth was found to occur at a temperature range of 15-40 °C, pH 6-10 and NaCl concentrations of 0-4%. The cell wall was found to contain meso-diaminopimelic acid and the whole cell sugars were identified as galactose, xylose and ribose. The predominant menaquinones were identified as MK-10(H) and MK-10(H). The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylinositol, phosphatidylglycerol, phosphoglycolipids and an unidentified glycolipid. The major fatty acids were identified as anteiso-C, iso-C, anteiso-C and iso-C. 16S rRNA gene sequence similarity studies showed that strain NEAU-C2 belongs to the genus Glycomyces with high sequence similarity to Glycomyces algeriensis NRRL B-16327 (99.0% sequence similarity). Some physiological and biochemical properties and low DNA-DNA relatedness values enabled the strain to be differentiated from closely related species of the genus Glycomyces. It is concluded that the isolate can be classified as representing a novel species of the genus Glycomyces, for which the name Glycomyces tritici is proposed. The type strain is NEAU-C2 (= DSM 104644 = CGMCC 4.7410).